Emerging and evolving concepts in gene essentiality
Gene essentiality is a founding concept of genetics with important implications in both
fundamental and applied research. Multiple screens have been performed over the years in …
fundamental and applied research. Multiple screens have been performed over the years in …
Antibiotics for emerging pathogens
MA Fischbach, CT Walsh - Science, 2009 - science.org
Antibiotic-resistant strains of pathogenic bacteria are increasingly prevalent in hospitals and
the community. New antibiotics are needed to combat these bacterial pathogens, but …
the community. New antibiotics are needed to combat these bacterial pathogens, but …
[PDF][PDF] Functional profiling of a Plasmodium genome reveals an abundance of essential genes
The genomes of malaria parasites contain many genes of unknown function. To assist drug
development through the identification of essential genes and pathways, we have measured …
development through the identification of essential genes and pathways, we have measured …
[PDF][PDF] Cas13b is a type VI-B CRISPR-associated RNA-guided RNase differentially regulated by accessory proteins Csx27 and Csx28
AA Smargon, DBT Cox, NK Pyzocha, K Zheng… - Molecular cell, 2017 - cell.com
CRISPR-Cas adaptive immune systems defend microbes against foreign nucleic acids via
RNA-guided endonucleases. Using a computational sequence database mining approach …
RNA-guided endonucleases. Using a computational sequence database mining approach …
Comprehensive Essentiality Analysis of the Mycobacterium tuberculosis Genome via Saturating Transposon Mutagenesis
MA DeJesus, ER Gerrick, W Xu, SW Park, JE Long… - MBio, 2017 - Am Soc Microbiol
For decades, identifying the regions of a bacterial chromosome that are necessary for
viability has relied on mapping integration sites in libraries of random transposon mutants to …
viability has relied on mapping integration sites in libraries of random transposon mutants to …
DEG 15, an update of the Database of Essential Genes that includes built-in analysis tools
Essential genes refer to genes that are required by an organism to survive under specific
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …
conditions. Studies of the minimal-gene-set for bacteria have elucidated fundamental …
Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system
The ability to artificially control transcription is essential both to the study of gene function
and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA …
and to the construction of synthetic gene networks with desired properties. Cas9 is an RNA …
DEG 10, an update of the database of essential genes that includes both protein-coding genes and noncoding genomic elements
The combination of high-density transposon-mediated mutagenesis and high-throughput
sequencing has led to significant advancements in research on essential genes, resulting in …
sequencing has led to significant advancements in research on essential genes, resulting in …
[HTML][HTML] Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance
To fully exploit the microbial genome resources, a high-throughput experimental platform is
needed to associate genes with phenotypes at the genome level. We present here a novel …
needed to associate genes with phenotypes at the genome level. We present here a novel …
EcoCyc: fusing model organism databases with systems biology
IM Keseler, A Mackie, M Peralta-Gil… - Nucleic acids …, 2013 - academic.oup.com
Abstract EcoCyc (http://EcoCyc. org) is a model organism database built on the genome
sequence of Escherichia coli K-12 MG1655. Expert manual curation of the functions of …
sequence of Escherichia coli K-12 MG1655. Expert manual curation of the functions of …