Discovery of diverse CRISPR-Cas systems and expansion of the genome engineering toolbox
CRISPR systems mediate adaptive immunity in bacteria and archaea through diverse
effector mechanisms and have been repurposed for versatile applications in therapeutics …
effector mechanisms and have been repurposed for versatile applications in therapeutics …
Applications of CRISPR/Cas13-based RNA editing in plants
The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR-
associated (Cas) system is widely used as a genome-editing tool in various organisms …
associated (Cas) system is widely used as a genome-editing tool in various organisms …
High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral effects
CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …
CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells
CRISPR/Cas13 effectors have garnered increasing attention as easily customizable tools for
detecting and depleting RNAs of interest. Near perfect complementarity between a target …
detecting and depleting RNAs of interest. Near perfect complementarity between a target …
Targeted gene silencing in the nervous system with CRISPR-Cas13
Cas13 nucleases are a class of programmable RNA-targeting CRISPR effector proteins that
are capable of silencing target gene expression in mammalian cells. Here, we demonstrate …
are capable of silencing target gene expression in mammalian cells. Here, we demonstrate …
[HTML][HTML] CRISPR-Cas13d induces efficient mRNA knockdown in animal embryos
G Kushawah, L Hernandez-Huertas, JAN Del Prado… - Developmental Cell, 2020 - cell.com
Early embryonic development is driven exclusively by maternal gene products deposited
into the oocyte. Although critical in establishing early developmental programs, maternal …
into the oocyte. Although critical in establishing early developmental programs, maternal …
A versatile toolkit for CRISPR-Cas13-based RNA manipulation in Drosophila
N Huynh, N Depner, R Larson, K King-Jones - Genome Biology, 2020 - Springer
Advances in CRISPR technology have immensely improved our ability to manipulate nucleic
acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further …
acids, and the recent discovery of the RNA-targeting endonuclease Cas13 adds even further …
Precise genome engineering in Drosophila using prime editing
JA Bosch, G Birchak… - Proceedings of the …, 2021 - National Acad Sciences
Precise genome editing is a valuable tool to study gene function in model organisms. Prime
editing, a precise editing system developed in mammalian cells, does not require double …
editing, a precise editing system developed in mammalian cells, does not require double …
[HTML][HTML] The double life of CRISPR–Cas13
JF Bot, J van der Oost, N Geijsen - Current Opinion in Biotechnology, 2022 - Elsevier
Highlights•CRISPR–Cas13 is potentially capable of collateral RNA cleavage in eukaryotic
cells.•Target RNA levels, cell size, and cell-type-specific properties may influence the …
cells.•Target RNA levels, cell size, and cell-type-specific properties may influence the …
Single-base tiled screen unveils design principles of PspCas13b for potent and off-target-free RNA silencing
The development of precise RNA-editing tools is essential for the advancement of RNA
therapeutics. CRISPR (clustered regularly interspaced short palindromic repeats) …
therapeutics. CRISPR (clustered regularly interspaced short palindromic repeats) …