Eukaryotic DNA polymerases
R Jain, AK Aggarwal, O Rechkoblit - Current opinion in structural biology, 2018 - Elsevier
Highlights•Humans encode 16 bone-fide DNA polymerases.•Replicative DNA polymerases
are emerging as highly flexible and dynamic in their interactions.•Mutations in replicative …
are emerging as highly flexible and dynamic in their interactions.•Mutations in replicative …
Recent developments in molecular sensor designs for inorganic pyrophosphate detection and biological imaging
Inorganic pyrophosphate (PPi) is a key anion which plays a crucial role in an extensive
range of biological and ecological processes. PPi is involved in many physiological …
range of biological and ecological processes. PPi is involved in many physiological …
[HTML][HTML] Cryo-EM structure of an extended SARS-CoV-2 replication and transcription complex reveals an intermediate state in cap synthesis
Summary Transcription of SARS-CoV-2 mRNA requires sequential reactions facilitated by
the replication and transcription complex (RTC). Here, we present a structural snapshot of …
the replication and transcription complex (RTC). Here, we present a structural snapshot of …
Nanoplasmonic amplification in microfluidics enables accelerated colorimetric quantification of nucleic acid biomarkers from pathogens
Deployment of nucleic acid amplification assays for diagnosing pathogens in point-of-care
settings is a challenge due to lengthy preparatory steps. We present a molecular diagnostic …
settings is a challenge due to lengthy preparatory steps. We present a molecular diagnostic …
A prebiotic basis for ATP as the universal energy currency
ATP is universally conserved as the principal energy currency in cells, driving metabolism
through phosphorylation and condensation reactions. Such deep conservation suggests that …
through phosphorylation and condensation reactions. Such deep conservation suggests that …
Metal/ADP complexes promote phosphorylation of ribonucleotides
Under enzyme catalysis, adenosine triphosphate (ATP) transfers a phosphoryl group to
canonical ribonucleotide diphosphates (NDPs) to form ribonucleotide triphosphates (NTPs) …
canonical ribonucleotide diphosphates (NDPs) to form ribonucleotide triphosphates (NTPs) …
Vitamin B12 may inhibit RNA‐dependent‐RNA polymerase activity of nsp12 from the SARS‐CoV‐2 virus
N Narayanan, DT Nair - IUBMB life, 2020 - Wiley Online Library
SARS‐CoV‐2 is the causative agent for the ongoing COVID19 pandemic, and this virus
belongs to the Coronaviridae family. Like other members of this family, the virus possesses a …
belongs to the Coronaviridae family. Like other members of this family, the virus possesses a …
Two-metal-ion catalysis: Inhibition of DNA polymerase activity by a third divalent metal ion
J Wang, WH Konigsberg - Frontiers in Molecular Biosciences, 2022 - frontiersin.org
Almost all DNA polymerases (pols) exhibit bell-shaped activity curves as a function of both
pH and Mg2+ concentration. The pol activity is reduced when the pH deviates from the …
pH and Mg2+ concentration. The pol activity is reduced when the pH deviates from the …
The ambivalent role of water at the origins of life
A do Nascimento Vieira, K Kleinermanns… - FEBS …, 2020 - Wiley Online Library
Life as we know it would not exist without water. However, water molecules not only serve as
a solvent and reactant but can also promote hydrolysis, which counteracts the formation of …
a solvent and reactant but can also promote hydrolysis, which counteracts the formation of …
Analysis and validation of silica-immobilised BST polymerase in loop-mediated isothermal amplification (LAMP) for malaria diagnosis
Bacillus stearothermophilus large fragment (BSTLF) DNA polymerase is reported, isolated
on silica via a fused R5 silica-affinity peptide and used in nucleic acid diagnostics. mCherry …
on silica via a fused R5 silica-affinity peptide and used in nucleic acid diagnostics. mCherry …