SMC (structural maintenance of chromosomes) complexes—which include condensin,
cohesin and the SMC5–SMC6 complex—are major components of chromosomes in all …

When fully extended, one copy of the three billion base pair human genome reaches a
length of over two meters. Yet, it must be packaged into the nucleus of a cell with an average …

In eukaryotes, higher-order chromatin organization is spatiotemporally regulated as
domains, for various cellular functions. However, their physical nature in living cells remains …

Eukaryotic chromatin is highly condensed but dynamically accessible to regulation and
organized into subdomains. We demonstrate that reconstituted chromatin undergoes …

INTRODUCTION During mitosis, cells compact their chromosomes into dense rod-shaped
structures to ensure their reliable transmission to daughter cells. Our work explores how …

INTRODUCTION In human cells, 2 m of DNA are compacted in the nucleus through
assembly with histones and other proteins into chromatin structures, megabase three …

We recently used in situ Hi-C to create kilobase-resolution 3D maps of mammalian
genomes. Here, we combine these maps with new Hi-C, microscopy, and genome-editing …

Nucleosomes help structure chromosomes by compacting DNA into fibers. To gain insight
into how nucleosomes are arranged in vivo, we combined quantitative super-resolution …

We describe a Hi-C-based method, Micro-C, in which micrococcal nuclease is used instead
of restriction enzymes to fragment chromatin, enabling nucleosome resolution chromosome …

Whereas the core nucleosome is thought to serve as a packaging device for the coiling and
contraction in length of genomic DNA, we suggest that it serves primarily in the regulation of …