Host-directed antiviral therapy
Antiviral drugs have traditionally been developed by directly targeting essential viral
components. However, this strategy often fails due to the rapid generation of drug-resistant …
components. However, this strategy often fails due to the rapid generation of drug-resistant …
CRISPR-Cas biology and its application to infectious diseases
JR Strich, DS Chertow - Journal of clinical microbiology, 2019 - Am Soc Microbiol
Infectious diseases remain a global threat contributing to excess morbidity and death
annually, with the persistent potential for destabilizing pandemics. Improved understanding …
annually, with the persistent potential for destabilizing pandemics. Improved understanding …
Genome-wide CRISPR screen identifies RACK1 as a critical host factor for flavivirus replication
Cellular factors have important roles in all facets of the flavivirus replication cycle.
Deciphering viral-host protein interactions is essential for understanding the flavivirus life …
Deciphering viral-host protein interactions is essential for understanding the flavivirus life …
A CRISPR activation screen identifies genes that protect against Zika virus infection
Zika virus (ZIKV) is an arthropod-borne emerging pathogen causing febrile illness. ZIKV is
associated Guillain-Barré syndrome and other neurological complications. Infection during …
associated Guillain-Barré syndrome and other neurological complications. Infection during …
Endoplasmic reticulum-associated degradation controls virus protein homeostasis, which is required for flavivirus propagation
K Tabata, M Arakawa, K Ishida, M Kobayashi… - Journal of …, 2021 - Am Soc Microbiol
Many positive-stranded RNA viruses encode polyproteins from which viral proteins are
generated by processing the polyproteins. This system produces an equal amount of each …
generated by processing the polyproteins. This system produces an equal amount of each …
West Nile virus-inclusive single-cell RNA sequencing reveals heterogeneity in the type I interferon response within single cells
JT O'Neal, AA Upadhyay, A Wolabaugh… - Journal of …, 2019 - Am Soc Microbiol
West Nile virus (WNV) is a neurotropic mosquito-borne flavivirus of global importance.
Neuroinvasive WNV infection results in encephalitis and can lead to prolonged neurological …
Neuroinvasive WNV infection results in encephalitis and can lead to prolonged neurological …
Pooled lentiviral CRISPR-Cas9 screens for functional genomics in mammalian cells
CRISPR-Cas9 technology provides a simple way to introduce targeted mutations into
mammalian cells to induce loss-of-function phenotypes. The CRISPR-Cas9 system has now …
mammalian cells to induce loss-of-function phenotypes. The CRISPR-Cas9 system has now …
Genome-scale CRISPR screening reveals host factors required for ribosome formation and viral replication
Viruses are known to co-opt host machinery for translation initiation, but less is known about
which host factors are required for the formation of ribosomes used to synthesize viral …
which host factors are required for the formation of ribosomes used to synthesize viral …
Upregulated expression of the antioxidant sestrin 2 identified by transcriptomic analysis of Japanese encephalitis virus-infected SH-SY5Y neuroblastoma cells
Japanese encephalitis virus (JEV) exerts a profound burden of viral encephalitis. We have
investigated the differentially expressed transcripts in the neuronal transcriptome during JEV …
investigated the differentially expressed transcripts in the neuronal transcriptome during JEV …
A disintegrin and metalloproteinase 9 domain (ADAM9) is a major susceptibility factor in the early stages of encephalomyocarditis virus infection
Encephalomyocarditis virus (EMCV) is a picornavirus that produces lytic infections in murine
and human cells. Employing a genome-wide CRISPR-Cas9 knockout screen to find host …
and human cells. Employing a genome-wide CRISPR-Cas9 knockout screen to find host …