Data‐independent acquisition‐based SWATH‐MS for quantitative proteomics: a tutorial

C Ludwig, L Gillet, G Rosenberger, S Amon… - Molecular systems …, 2018 - embopress.org
Many research questions in fields such as personalized medicine, drug screens or systems
biology depend on obtaining consistent and quantitatively accurate proteomics data from …

Advances in data‐independent acquisition mass spectrometry towards comprehensive digital proteome landscape

RB Kitata, JC Yang, YJ Chen - Mass spectrometry reviews, 2023 - Wiley Online Library
The data‐independent acquisition mass spectrometry (DIA‐MS) has rapidly evolved as a
powerful alternative for highly reproducible proteome profiling with a unique strength of …

NAD+ repletion improves mitochondrial and stem cell function and enhances life span in mice

H Zhang, D Ryu, Y Wu, K Gariani, X Wang, P Luan… - Science, 2016 - science.org
Adult stem cells (SCs) are essential for tissue maintenance and regeneration yet are
susceptible to senescence during aging. We demonstrate the importance of the amount of …

Integrated proteogenomic characterization of human high-grade serous ovarian cancer

H Zhang, T Liu, Z Zhang, SH Payne, B Zhang… - Cell, 2016 - cell.com
To provide a detailed analysis of the molecular components and underlying mechanisms
associated with ovarian cancer, we performed a comprehensive mass-spectrometry-based …

Multi-omic measurements of heterogeneity in HeLa cells across laboratories

Y Liu, Y Mi, T Mueller, S Kreibich, EG Williams… - Nature …, 2019 - nature.com
Reproducibility in research can be compromised by both biological and technical variation,
but most of the focus is on removing the latter. Here we investigate the effects of biological …

Optimization of experimental parameters in data-independent mass spectrometry significantly increases depth and reproducibility of results

R Bruderer, OM Bernhardt, T Gandhi, Y Xuan… - Molecular & Cellular …, 2017 - ASBMB
Comprehensive, reproducible and precise analysis of large sample cohorts is one of the key
objectives of quantitative proteomics. Here, we present an implementation of data …

Mass spectrometry‐based protein–protein interaction networks for the study of human diseases

AL Richards, M Eckhardt, NJ Krogan - Molecular systems biology, 2021 - embopress.org
A better understanding of the molecular mechanisms underlying disease is key for
expediting the development of novel therapeutic interventions. Disease mechanisms are …

Multi-laboratory assessment of reproducibility, qualitative and quantitative performance of SWATH-mass spectrometry

BC Collins, CL Hunter, Y Liu, B Schilling… - Nature …, 2017 - nature.com
Quantitative proteomics employing mass spectrometry is an indispensable tool in life
science research. Targeted proteomics has emerged as a powerful approach for …

Meningeal γδ T cell–derived IL-17 controls synaptic plasticity and short-term memory

M Ribeiro, HC Brigas, M Temido-Ferreira… - Science …, 2019 - science.org
The notion of “immune privilege” of the brain has been revised to accommodate its
infiltration, at steady state, by immune cells that participate in normal neurophysiology …

[HTML][HTML] A human interactome in three quantitative dimensions organized by stoichiometries and abundances

MY Hein, NC Hubner, I Poser, J Cox, N Nagaraj… - Cell, 2015 - cell.com
The organization of a cell emerges from the interactions in protein networks. The
interactome is critically dependent on the strengths of interactions and the cellular …