Polarization microscopy: from ensemble structural imaging to single-molecule 3D orientation and localization microscopy

S Brasselet, MA Alonso - Optica, 2023 - opg.optica.org
Optical contrasts in microscopy are sensitive to light polarization, whose interaction with
molecular dipoles provides an important lever for probing molecular orientation. Polarization …

Extending single-molecule microscopy using optical Fourier processing

AS Backer, WE Moerner - The Journal of Physical Chemistry B, 2014 - ACS Publications
This article surveys the recent application of optical Fourier processing to the long-
established but still expanding field of single-molecule imaging and microscopy. A variety of …

Distance-dependent plasmon resonant coupling between a gold nanoparticle and gold film

JJ Mock, RT Hill, A Degiron, S Zauscher, A Chilkoti… - Nano …, 2008 - ACS Publications
We present an experimental analysis of the plasmonic scattering properties of gold
nanoparticles controllably placed nanometers away from a gold metal film. We show that the …

High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes

K Zhanghao, W Liu, M Li, Z Wu, X Wang, X Chen… - Nature …, 2020 - nature.com
Lipid membranes are found in most intracellular organelles, and their heterogeneities play
an essential role in regulating the organelles' biochemical functionalities. Here we report a …

Photoluminescence of carbon nanodots: dipole emission centers and electron–phonon coupling

S Ghosh, AM Chizhik, N Karedla, MO Dekaliuk… - Nano …, 2014 - ACS Publications
Inorganic carbon nanomaterials, also called carbon nanodots, exhibit a strong
photoluminescence with unusual properties and, thus, have been the focus of intense …

Simultaneous, accurate measurement of the 3D position and orientation of single molecules

MP Backlund, MD Lew, AS Backer… - Proceedings of the …, 2012 - National Acad Sciences
Recently, single molecule-based superresolution fluorescence microscopy has surpassed
the diffraction limit to improve resolution to the order of 20 nm or better. These methods …

Particle tracking of nanoparticles in soft matter

KA Rose, M Molaei, MJ Boyle, D Lee… - Journal of Applied …, 2020 - pubs.aip.org
Recent advances in optical microscopy instrumentation and processing techniques have led
to imaging that both breaks the diffraction barrier and enables sub-pixel resolution. This …

Polymers and single molecule fluorescence spectroscopy, what can we learn?

D Wöll, E Braeken, A Deres, FC De Schryver… - Chemical Society …, 2009 - pubs.rsc.org
This tutorial review summarizes the most important results and developments in the field of
polymer science by means of single molecule fluorescence spectroscopy (SMFS) at ambient …

Metal-induced energy transfer for live cell nanoscopy

AI Chizhik, J Rother, I Gregor, A Janshoff… - Nature Photonics, 2014 - nature.com
The discovery of Förster resonance energy transfer (FRET) has revolutionized our ability to
measure inter-and intramolecular distances on the nanometre scale using fluorescence …

Molecular orientation affects localization accuracy in superresolution far-field fluorescence microscopy

J Engelhardt, J Keller, P Hoyer, M Reuss, T Staudt… - Nano …, 2011 - ACS Publications
We investigate the cooperative effect of molecular tilt and defocus on fluorophore
localization by centroid calculation in far-field superresolution microscopy based on …