[HTML][HTML] Microbiome definition re-visited: old concepts and new challenges

G Berg, D Rybakova, D Fischer, T Cernava… - Microbiome, 2020 - Springer
The field of microbiome research has evolved rapidly over the past few decades and has
become a topic of great scientific and public interest. As a result of this rapid growth in …

[HTML][HTML] Unraveling metagenomics through long-read sequencing: A comprehensive review

C Kim, M Pongpanich, T Porntaveetus - Journal of Translational Medicine, 2024 - Springer
The study of microbial communities has undergone significant advancements, starting from
the initial use of 16S rRNA sequencing to the adoption of shotgun metagenomics. However …

[HTML][HTML] Genetic markers for metabarcoding of freshwater microalgae

E Kezlya, N Tseplik, M Kulikovskiy - Biology, 2023 - mdpi.com
Simple Summary The metabarcoding approach is widely used for studying the diversity and
distribution of freshwater microalgae and for routine biomonitoring. Due to microalgae being …

[HTML][HTML] Comparative analyses of the V4 and V9 regions of 18S rDNA for the extant eukaryotic community using the Illumina platform

J Choi, JS Park - Scientific Reports, 2020 - nature.com
Illumina sequencing is a representative tool for understanding the massive diversity of
microbial eukaryotes in natural ecosystems. Here, we investigated the eukaryotic community …

Overlapping community compositions of gut and fecal microbiomes in lab-reared and field-collected German cockroaches

ML Kakumanu, JM Maritz, JM Carlton… - Applied and …, 2018 - Am Soc Microbiol
ABSTRACT German cockroaches, Blattella germanica (Blattodea: Ectobiidae), are human
commensals that move freely between food and waste, disseminating bacteria, including …

Microbiological quality of irrigation water for cultivation of fruits and vegetables: An overview of available guidelines, water testing strategies and some factors that …

O Alegbeleye, AS Sant'Ana - Environmental Research, 2023 - Elsevier
Contaminated irrigation water is among many potential vehicles of human pathogens to food
plants, constituting significant public health risks especially for the fresh produce category …

[HTML][HTML] VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages

LA Owens, S Friant, B Martorelli Di Genova… - Nature …, 2024 - nature.com
Protocols for characterizing taxonomic assemblages by deep sequencing of short DNA
barcode regions (metabarcoding) have revolutionized our understanding of microbial …

[HTML][HTML] Improved 18S and 28S rDNA primer sets for NGS-based parasite detection

A Kounosu, K Murase, A Yoshida, H Maruyama… - Scientific reports, 2019 - nature.com
The development and application of next-generation sequencing (NGS) have enabled
comprehensive analyses of the microbial community through extensive parallel sequencing …

Measurements of soil protist richness and community composition are influenced by primer pair, annealing temperature, and bioinformatics choices

RL Mau, M Hayer, AM Purcell, S Geisen… - Applied and …, 2024 - journals.asm.org
Protists are a diverse and understudied group of microbial eukaryotic organisms especially
in terrestrial environments. Advances in molecular methods are increasing our …

Identification of eukaryotic microorganisms with 18S rRNA next-generation sequencing in wastewater treatment plants, with a more targeted NGS approach required …

A Zahedi, TL Greay, A Paparini, KL Linge, CA Joll… - Water research, 2019 - Elsevier
While some microbial eukaryotes can improve effluent quality in wastewater treatment plants
(WWTPs), eukaryotic waterborne pathogens are a threat to public health. This study aimed …