Proliferation, survival and metabolism: the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination
JSL Yu, W Cui - Development, 2016 - journals.biologists.com
Phosphatidylinositide 3 kinases (PI3Ks) and their downstream mediators AKT and
mammalian target of rapamycin (mTOR) constitute the core components of the …
mammalian target of rapamycin (mTOR) constitute the core components of the …
Differentiation of human embryonic stem cells and induced pluripotent stem cells to cardiomyocytes: a methods overview
Since human embryonic stem cells were first differentiated to beating cardiomyocytes a
decade ago, interest in their potential applications has increased exponentially. This has …
decade ago, interest in their potential applications has increased exponentially. This has …
Directed cardiomyocyte differentiation from human pluripotent stem cells by modulating Wnt/β-catenin signaling under fully defined conditions
The protocol described here efficiently directs human pluripotent stem cells (hPSCs) to
functional cardiomyocytes in a completely defined, growth factor–and serum-free system by …
functional cardiomyocytes in a completely defined, growth factor–and serum-free system by …
Differentiation of cardiomyocytes and generation of human engineered heart tissue
K Breckwoldt, D Letuffe-Brenière, I Mannhardt… - Nature protocols, 2017 - nature.com
Since the advent of the generation of human induced pluripotent stem cells (hiPSCs),
numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and …
numerous protocols have been developed to differentiate hiPSCs into cardiomyocytes and …
Production of de novo cardiomyocytes: human pluripotent stem cell differentiation and direct reprogramming
PW Burridge, G Keller, JD Gold, JC Wu - Cell stem cell, 2012 - cell.com
Cardiovascular disease is a leading cause of death worldwide. The limited capability of
heart tissue to regenerate has prompted methodological developments for creating de novo …
heart tissue to regenerate has prompted methodological developments for creating de novo …
Extracellular matrix promotes highly efficient cardiac differentiation of human pluripotent stem cells: the matrix sandwich method
J Zhang, M Klos, GF Wilson, AM Herman… - Circulation …, 2012 - Am Heart Assoc
Rationale: Cardiomyocytes (CMs) differentiated from human pluripotent stem cells (PSCs)
are increasingly being used for cardiovascular research, including disease modeling, and …
are increasingly being used for cardiovascular research, including disease modeling, and …
Opportunities and challenges in cardiac tissue engineering from an analysis of two decades of advances
Engineered human cardiac tissues facilitate progress in regenerative medicine, disease
modelling and drug development. In this Perspective, we reflect on the most notable …
modelling and drug development. In this Perspective, we reflect on the most notable …
Mitochondria and metabolic transitions in cardiomyocytes: lessons from development for stem cell-derived cardiomyocytes
JC Garbern, RT Lee - Stem Cell Research & Therapy, 2021 - Springer
Current methods to differentiate cardiomyocytes from human pluripotent stem cells (PSCs)
inadequately recapitulate complete development and result in PSC-derived cardiomyocytes …
inadequately recapitulate complete development and result in PSC-derived cardiomyocytes …
[HTML][HTML] A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming
The methods for the culture and cardiomyocyte differentiation of human embryonic stem
cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex …
cells, and later human induced pluripotent stem cells (hiPSC), have moved from a complex …
A universal system for highly efficient cardiac differentiation of human induced pluripotent stem cells that eliminates interline variability
PW Burridge, S Thompson, MA Millrod, S Weinberg… - PloS one, 2011 - journals.plos.org
Background The production of cardiomyocytes from human induced pluripotent stem cells
(hiPSC) holds great promise for patient-specific cardiotoxicity drug testing, disease …
(hiPSC) holds great promise for patient-specific cardiotoxicity drug testing, disease …