The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Today, Fmoc SPPS is the method of choice for peptide synthesis. Very‐high‐quality Fmoc
building blocks are available at low cost because of the economies of scale arising from …

Bioconjugation methodologies have proven to play a central enabling role in the recent
development of biotherapeutics and chemical biology approaches. Recent endeavours in …

Methods to prepare proteins that include a specific modification at a desired position are
essential for understanding their cellular functions and physical properties in living systems …

This review article highlights the strategies to successfully perform an efficient solid-phase
synthesis of complex peptides including posttranslational modifications, fluorescent labels …

The translation of biological glycosylation in humans to the clinical applications involves
systematic studies using homogeneous samples of oligosaccharides and glycoconjugates …

Nature has developed a plethora of protein machinery to operate and maintain nearly every
task of cellular life. These processes are tightly regulated via post‐expression modifications …

Organic chemistry allows for the modification and chemical preparation of protein analogues
for various studies. The thiolate side chain of the Cys residue has been a key functionality in …

Facilitating the process of chemical protein synthesis is an important goal in order to enable
the efficient preparation of large and novel protein analogues. Native chemical ligation …

In this article, Ser/Thr ligationon/off has been realized to enable N-to-C successive peptide
ligations using a salicylaldehyde semicarbazone (SALoff) group by in situ activation with …