Synthetic DNA synthesis and assembly: putting the synthetic in synthetic biology
RA Hughes, AD Ellington - Cold Spring Harbor …, 2017 - cshperspectives.cshlp.org
The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …
circuits, and even entire genomes by gene synthesis methods has become an enabling …
Large-scale de novo DNA synthesis: technologies and applications
For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …
understand and engineer biology. Here we summarize methods and caveats for the de novo …
Synthetic biology—putting engineering into biology
M Heinemann, S Panke - Bioinformatics, 2006 - academic.oup.com
Synthetic biology is interpreted as the engineering-driven building of increasingly complex
biological entities for novel applications. Encouraged by progress in the design of artificial …
biological entities for novel applications. Encouraged by progress in the design of artificial …
Genome engineering
PA Carr, GM Church - Nature biotechnology, 2009 - nature.com
For more than 50 years, those engineering genetic material have pursued increasingly
challenging targets. During that time, the tools and resources available to the genetic …
challenging targets. During that time, the tools and resources available to the genetic …
[HTML][HTML] Java web tools for PCR, in silico PCR, and oligonucleotide assembly and analysis
The polymerase chain reaction is fundamental to molecular biology and is the most
important practical molecular technique for the research laboratory. We have developed and …
important practical molecular technique for the research laboratory. We have developed and …
Fumarase: a mitochondrial metabolic enzyme and a cytosolic/nuclear component of the DNA damage response
O Yogev, O Yogev, E Singer, E Shaulian… - PLoS …, 2010 - journals.plos.org
In eukaryotes, fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme in
the mitochondrial matrix. However, conserved from yeast to humans is a cytosolic …
the mitochondrial matrix. However, conserved from yeast to humans is a cytosolic …
PCR-based accurate synthesis of long DNA sequences
AS Xiong, QH Yao, RH Peng, H Duan, X Li, HQ Fan… - Nature protocols, 2006 - nature.com
Here we describe a simple and rapid method for assembly and PCR-based accurate
synthesis (PAS) of long DNA sequences. The PAS protocol involves the following five …
synthesis (PAS) of long DNA sequences. The PAS protocol involves the following five …
In vitro synthesis of gene-length single-stranded DNA
R Veneziano, TR Shepherd, S Ratanalert, L Bellou… - Scientific reports, 2018 - nature.com
Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with
reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum …
reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum …
Functional hybrid rubisco enzymes with plant small subunits and algal large subunits: Engineered rbcS cDNA for expression in chlamydomonas*♦
T Genkov, M Meyer, H Griffiths, RJ Spreitzer - Journal of Biological …, 2010 - ASBMB
There has been much interest in the chloroplast-encoded large subunit of ribulose-1, 5-
bisphosphate carboxylase/oxygenase (Rubisco) as a target for engineering an increase in …
bisphosphate carboxylase/oxygenase (Rubisco) as a target for engineering an increase in …
Comparison of five protein engineering strategies for stabilizing an α/β-hydrolase
A review of the previous stabilization of α/β-hydrolase fold enzymes revealed many different
strategies, but no comparison of strategies on the same enzyme. For this reason, we …
strategies, but no comparison of strategies on the same enzyme. For this reason, we …