The chemical synthesis of DNA oligonucleotides and their assembly into synthons, genes,
circuits, and even entire genomes by gene synthesis methods has become an enabling …

For over 60 years, the synthetic production of new DNA sequences has helped researchers
understand and engineer biology. Here we summarize methods and caveats for the de novo …

Synthetic biology is interpreted as the engineering-driven building of increasingly complex
biological entities for novel applications. Encouraged by progress in the design of artificial …

For more than 50 years, those engineering genetic material have pursued increasingly
challenging targets. During that time, the tools and resources available to the genetic …

The polymerase chain reaction is fundamental to molecular biology and is the most
important practical molecular technique for the research laboratory. We have developed and …

In eukaryotes, fumarase (FH in human) is a well-known tricarboxylic-acid-cycle enzyme in
the mitochondrial matrix. However, conserved from yeast to humans is a cytosolic …

Here we describe a simple and rapid method for assembly and PCR-based accurate
synthesis (PAS) of long DNA sequences. The PAS protocol involves the following five …

Single-stranded DNA (ssDNA) increases the likelihood of homology directed repair with
reduced cellular toxicity. However, ssDNA synthesis strategies are limited by the maximum …

There has been much interest in the chloroplast-encoded large subunit of ribulose-1, 5-
bisphosphate carboxylase/oxygenase (Rubisco) as a target for engineering an increase in …

A review of the previous stabilization of α/β-hydrolase fold enzymes revealed many different
strategies, but no comparison of strategies on the same enzyme. For this reason, we …