Differential scanning fluorimetry (DSF) is an accessible, rapid, and economical biophysical
technique that has seen many applications over the years, ranging from protein folding state …

Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …

Escherichia coli is one of the organisms of choice for the production of recombinant proteins.
Its use as a cell factory is well-established and it has become the most popular expression …

Antimicrobial peptides (AMPs) found in a wide range of animal, insect, and plant species are
host defense peptides forming an integral part of their innate immunity. Although the exact …

Inclusion bodies produced in Escherichia coli are composed of densely packed denatured
protein molecules in the form of particles. Refolding of inclusion body proteins into bioactive …

In order for any biological system to function effectively, it is essential to avoid the inherent
tendency of proteins to aggregate and form potentially harmful deposits,,,. In each of the …

The secretory production of recombinant proteins by the Gram-negative bacterium
Escherichia coli has several advantages over intracellular production as inclusion bodies. In …

Protein folding is a biophysical process by which proteins reach a specific three-dimensional
structure. The amino acid sequence of a polypeptide chain contains all the information …

Ever since lysozyme was discovered by Fleming in 1922, this protein has emerged as a
model for investigations on protein structure and function. Over the years, several high …

Protein aggregation is an ordinary consequence of thermal stress. In recombinant bacteria,
the over-expression of plasmid-encoded genes triggers transcription of heat-shock genes …