Advances and challenges in small‐molecule DNA aptamer isolation, characterization, and sensor development
Aptamers are short oligonucleotides isolated in vitro from randomized libraries that can bind
to specific molecules with high affinity, and offer a number of advantages relative to …
to specific molecules with high affinity, and offer a number of advantages relative to …
Investigations on the interface of nucleic acid aptamers and binding targets
S Cai, J Yan, H Xiong, Y Liu, D Peng, Z Liu - Analyst, 2018 - pubs.rsc.org
Nucleic acid aptamers are single-stranded DNA or RNA of 20–100 nucleotides in length that
have attracted substantial scientific interest due to their ability to specifically bind to target …
have attracted substantial scientific interest due to their ability to specifically bind to target …
Do aptamers always bind? The need for a multifaceted analytical approach when demonstrating binding affinity between aptamer and low molecular weight …
F Bottari, E Daems, AM de Vries… - Journal of the …, 2020 - ACS Publications
In this manuscript, we compare different analytical methodologies to validate or disprove the
binding capabilities of aptamer sequences. This was prompted by the lack of a universally …
binding capabilities of aptamer sequences. This was prompted by the lack of a universally …
Design and clinical developments of aptamer-drug conjugates for targeted cancer therapy
DH Kim, JM Seo, KJ Shin, SG Yang - Biomaterials Research, 2021 - Springer
Background Aptamer has been called “chemical antibody” which displays the specific affinity
to target molecules compared to that of antibodies and possesses several therapeutic …
to target molecules compared to that of antibodies and possesses several therapeutic …
Innovative engineering and sensing strategies for aptamer-based small-molecule detection
Aptamers are nucleic acid-based affinity reagents that have gained widespread attention as
biorecognition elements for the detection of targets such as ions, small molecules, and …
biorecognition elements for the detection of targets such as ions, small molecules, and …
Recent advances in electrochemical biosensing schemes using graphene and graphene-based nanocomposites
SK Vashist, JHT Luong - Carbon, 2015 - Elsevier
The last decade has witnessed tremendous advances in the use of graphene and graphene-
based nanocomposites for the fabrication of electrochemical (EC) sensors and biosensors …
based nanocomposites for the fabrication of electrochemical (EC) sensors and biosensors …
[HTML][HTML] Oligonucleotide aptamers: Recent advances in their screening, molecular conformation and therapeutic applications
X Xiao, H Li, L Zhao, Y Zhang, Z Liu - Biomedicine & Pharmacotherapy, 2021 - Elsevier
Aptamers are single stranded oligonucleotides with specific recognition and binding ability
to target molecules, which can be obtained by Systematic Evolution of Ligands by …
to target molecules, which can be obtained by Systematic Evolution of Ligands by …
Accelerating post-SELEX aptamer engineering using exonuclease digestion
The systematic evolution of ligands by exponential enrichment (SELEX) process enables the
isolation of aptamers from random oligonucleotide libraries. However, it is generally difficult …
isolation of aptamers from random oligonucleotide libraries. However, it is generally difficult …
New insights into a classic aptamer: binding sites, cooperativity and more sensitive adenosine detection
The DNA aptamer for adenosine (also for AMP and ATP) is a highly conserved sequence
that has recurred in a few selections. It it a widely used model aptamer for biosensor …
that has recurred in a few selections. It it a widely used model aptamer for biosensor …
Analysis of in vitro aptamer selection parameters
Nucleic acid aptamers are novel molecular recognition tools that offer many advantages
compared to their antibody and peptide-based counterparts. However, challenges …
compared to their antibody and peptide-based counterparts. However, challenges …