[HTML][HTML] Protein recovery from inclusion bodies of Escherichia coli using mild solubilization process
Formation of inclusion bodies in bacterial hosts poses a major challenge for large scale
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …
recovery of bioactive proteins. The process of obtaining bioactive protein from inclusion …
Bacterial inclusion bodies: a treasure trove of bioactive proteins
Recombinant proteins expressed as bacterial inclusion bodies (IBs) are now receiving
tremendous attention for many diverse applications in the areas of industrial and medical …
tremendous attention for many diverse applications in the areas of industrial and medical …
Bacterial inclusion bodies are industrially exploitable amyloids
A De Marco, N Ferrer-Miralles… - FEMS microbiology …, 2019 - academic.oup.com
Understanding the structure, functionalities and biology of functional amyloids is an issue of
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …
emerging interest. Inclusion bodies, namely protein clusters formed in recombinant bacteria …
Microbial production, molecular modification, and practical application of L-asparaginase: A review
Y Wang, W Xu, H Wu, W Zhang, C Guang… - International Journal of …, 2021 - Elsevier
Abstract L-Asparaginase (L-ASNase, EC 3.5. 1.1), an antitumor drug for acute lymphoblastic
leukemia (ALL) therapy, is widely used in the clinical field. Similarly, L-ASNase is also a …
leukemia (ALL) therapy, is widely used in the clinical field. Similarly, L-ASNase is also a …
Catalytically-active inclusion bodies—Carrier-free protein immobilizates for application in biotechnology and biomedicine
Bacterial inclusion bodies (IBs) consist of unfolded protein aggregates and represent
inactive waste products often accumulating during heterologous overexpression of …
inactive waste products often accumulating during heterologous overexpression of …
[HTML][HTML] Evaluation of scFv protein recovery from E. coli by in vitro refolding and mild solubilization process
Background The production of therapeutically active single chain variable fragment (scFv)
antibody is still challenging in E. coli due to the aggregation propensity of recombinant …
antibody is still challenging in E. coli due to the aggregation propensity of recombinant …
Critical overview of the main features and techniques used for the evaluation of the clinical applicability of L-asparaginase as a biopharmaceutical to treat blood …
TA Costa-Silva, IM Costa, HP Biasoto, GM Lima… - Blood Reviews, 2020 - Elsevier
L-asparaginase is an enzyme used as a biopharmaceutical to treat acute lymphoblastic
leukemia. Several adverse effects have been related to L-asparaginase use, so the scientific …
leukemia. Several adverse effects have been related to L-asparaginase use, so the scientific …
Glutaminase free L-asparaginase from Vibrio cholerae: heterologous expression, purification and biochemical characterization
R Radha, N Arumugam, SN Gummadi - International journal of biological …, 2018 - Elsevier
Abstract l-asparaginase is used as a pivotal agent in treatment of lympho proliferative
disorders and holds an excessive demand for food processing aids. Most of the l …
disorders and holds an excessive demand for food processing aids. Most of the l …
Molecular cloning, structural modeling and production of recombinant Aspergillus terreus L. asparaginase in Escherichia coli
Abstract l-Asparaginase (EC 3.5. 1.1) is an important medical enzyme that catalysis the
hydrolysis of l-asparagine to aspartic acid and ammonium. For over four decades l …
hydrolysis of l-asparagine to aspartic acid and ammonium. For over four decades l …
[HTML][HTML] Recombinant expression of insoluble enzymes in Escherichia coli: a systematic review of experimental design and its manufacturing implications
Recombinant enzyme expression in Escherichia coli is one of the most popular methods to
produce bulk concentrations of protein product. However, this method is often limited by the …
produce bulk concentrations of protein product. However, this method is often limited by the …