Stemness combines the ability of a cell to perpetuate its lineage, to give rise to differentiated
cells, and to interact with its environment to maintain a balance between quiescence …

Nonclinical testing has served as a foundation for evaluating potential risks and
effectiveness of investigational new drugs in humans. However, the current two‐dimensional …

Adult stem cell–based organoid technology is a versatile tool for the generation and long-
term maintenance of near-native 3D epithelial tissues in vitro. The generation of cancer …

Colorectal carcinoma represents a heterogeneous entity, with only a fraction of the tumours
responding to available therapies, requiring a better molecular understanding of the disease …

Patient-derived organoids resemble the biology of tissues and tumors, enabling ex vivo
modeling of human diseases. They have heterogeneous morphologies with unclear …

In nature, cells reside in tissues subject to complex cell–cell interactions, signals from
extracellular molecules and niche soluble and mechanical signaling. These …

Circulating tumor cells (CTCs) are a rare subset of cells found in the blood of patients with
solid tumors, which function as a seed for metastases. Cancer cells metastasize through the …

The recent advent of robust methods to grow human tissues as 3D organoids allows us to
recapitulate the 3D architecture of tumors in an in vitro setting and offers a new orthogonal …

Precision oncology presumes an accurate prediction of drug response on the basis of the
molecular profile of tumors. However, the extent to which patient-derived tumor organoids …

Traditional high-throughput drug screening in oncology routinely relies on two-dimensional
(2D) cell models, which inadequately recapitulate the physiologic context of cancer. Three …