The application of clustered regularly interspaced short palindromic repeats (CRISPR) for
genetic manipulation has revolutionized life science over the past few years. CRISPR was …

Since it was first recognized in bacteria and archaea as a mechanism for innate viral
immunity in the early 2010s, clustered regularly interspaced short palindromic repeats …

Many organisms evolved strategies to survive desiccation. Plant seeds protect dehydrated
embryos from various stressors and can lay dormant for millennia. Hydration is the key …

The rapid development of the CRISPR–Cas9,–Cas12a and–Cas12b genome editing
systems has greatly fuelled basic and translational plant research,,,,–. DNA targeting by …

Traditionally, generation of new plants with improved or desirable features has relied on
laborious and time-consuming breeding techniques. Genome-editing technologies have led …

The emergence of sequence-specific nucleases that enable genome editing is
revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome …

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein 9
(Cas9) genome editing system is a powerful tool for targeted gene modifications in a wide …

Fruits are major sources of essential nutrients and serve as staple foods in some areas of
the world. The increasing human population and changes in climate experienced worldwide …

The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …

CRISPR construct design is a key step in the practice of genome editing, which includes
identification of appropriate Cas proteins, design and selection of guide RNAs (gRNAs), and …