Recombinant protein (RP) production from Escherichia coli has been extensively studied to
find strategies for increasing product yields. The thermoinducible expression system is …

High‐value heterologous proteins produced in Escherichia coli that contain disulfide bonds
are almost invariably targeted to the periplasm via the Sec pathway as it, among other …

Changes in bacterial physiology caused by the combined action of the magnetic force and
microgravity were studied in Escherichia coli grown using a specially developed device …

Escherichia coli is a heavily used platform for the production of biotherapeutic and other
high‐value proteins, and a favored strategy is to export the protein of interest to the …

High-level production of recombinant proteins in industrial microorganisms is often limited
by the formation of misfolded proteins or protein aggregates, which consequently induce …

Nanobodies are single-domain antibody fragments that have garnered considerable use as
diagnostic and therapeutic agents as well as research tools. However, obtaining pure VHHs …

Iron is an essential element involved in various metabolic processes. The ferritin family of
proteins forms nanocage assembly and is involved in iron oxidation, storage, and …

The sigma H (σΗ) and sigma E (σE) subunits of Corynebacterium glutamicum RNA
polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) …

Identification of a single genetic target for microbial strain improvement is difficult due to the
complexity of the genetic regulatory network. Hence, a more practical approach is to identify …

Overproduction of the membrane proteins in Escherichia coli cells is a common approach to
obtain sufficient material for their functional and structural studies. However, the efficiency of …