Thermoinducible expression system for producing recombinant proteins in Escherichia coli: advances and insights
S Restrepo-Pineda, N O. Pérez… - FEMS Microbiology …, 2021 - academic.oup.com
Recombinant protein (RP) production from Escherichia coli has been extensively studied to
find strategies for increasing product yields. The thermoinducible expression system is …
find strategies for increasing product yields. The thermoinducible expression system is …
Highly efficient export of a disulfide‐bonded protein to the periplasm and medium by the Tat pathway using CyDisCo in Escherichia coli
K Arauzo‐Aguilera, MJ Saaranen… - …, 2023 - Wiley Online Library
High‐value heterologous proteins produced in Escherichia coli that contain disulfide bonds
are almost invariably targeted to the periplasm via the Sec pathway as it, among other …
are almost invariably targeted to the periplasm via the Sec pathway as it, among other …
Combined Impact of Magnetic Force and Spaceflight Conditions on Escherichia coli Physiology
PA Domnin, VA Parfenov, AS Kononikhin… - International Journal of …, 2022 - mdpi.com
Changes in bacterial physiology caused by the combined action of the magnetic force and
microgravity were studied in Escherichia coli grown using a specially developed device …
microgravity were studied in Escherichia coli grown using a specially developed device …
Escherichia coli “TatExpress” strains export several g/L human growth hormone to the periplasm by the Tat pathway
I Guerrero Montero, KL Richards… - Biotechnology and …, 2019 - Wiley Online Library
Escherichia coli is a heavily used platform for the production of biotherapeutic and other
high‐value proteins, and a favored strategy is to export the protein of interest to the …
high‐value proteins, and a favored strategy is to export the protein of interest to the …
[HTML][HTML] Suppressors of amyloid-β toxicity improve recombinant protein production in yeast by reducing oxidative stress and tuning cellular metabolism
High-level production of recombinant proteins in industrial microorganisms is often limited
by the formation of misfolded proteins or protein aggregates, which consequently induce …
by the formation of misfolded proteins or protein aggregates, which consequently induce …
Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control
JN Hennigan, R Menacho-Melgar, P Sarkar… - Metabolic …, 2024 - Elsevier
Nanobodies are single-domain antibody fragments that have garnered considerable use as
diagnostic and therapeutic agents as well as research tools. However, obtaining pure VHHs …
diagnostic and therapeutic agents as well as research tools. However, obtaining pure VHHs …
Bacterioferritin nanocage structures uncover the biomineralization process in ferritins
C Jobichen, T Ying Chong, R Rattinam, S Basak… - PNAS …, 2023 - academic.oup.com
Iron is an essential element involved in various metabolic processes. The ferritin family of
proteins forms nanocage assembly and is involved in iron oxidation, storage, and …
proteins forms nanocage assembly and is involved in iron oxidation, storage, and …
Overlapping SigH and SigE sigma factor regulons in Corynebacterium glutamicum
T Busche, H Dostálová, L Rucká, J Holátko… - Frontiers in …, 2023 - frontiersin.org
The sigma H (σΗ) and sigma E (σE) subunits of Corynebacterium glutamicum RNA
polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) …
polymerase belong to Group 4 of sigma factors, also called extracytoplasmic function (ECF) …
Improving the production of recombinant L-Asparaginase-II in Escherichia coli by co-expressing catabolite repressor activator (cra) gene
D Mittra, S Mahalik - Preparative Biochemistry & Biotechnology, 2024 - Taylor & Francis
Identification of a single genetic target for microbial strain improvement is difficult due to the
complexity of the genetic regulatory network. Hence, a more practical approach is to identify …
complexity of the genetic regulatory network. Hence, a more practical approach is to identify …
Increased Synthesis of a Magnesium Transporter MgtA During Recombinant Autotransporter Expression in Escherichia coli
LE Petrovskaya, RH Ziganshin, EA Kryukova… - Applied Biochemistry …, 2021 - Springer
Overproduction of the membrane proteins in Escherichia coli cells is a common approach to
obtain sufficient material for their functional and structural studies. However, the efficiency of …
obtain sufficient material for their functional and structural studies. However, the efficiency of …