The entropy-controlled strategy in self-assembling systems

X Zhang, X Dai, L Gao, D Xu, H Wan, Y Wang… - Chemical Society …, 2023 - pubs.rsc.org
Self-assembly of various building blocks has been considered as a powerful approach to
generate novel materials with tailorable structures and optimal properties. Understanding …

Enzyme fusions in biocatalysis: coupling reactions by pairing enzymes

FS Aalbers, MW Fraaije - ChemBioChem, 2019 - Wiley Online Library
One approach to bringing enzymes together for multienzyme biocatalysis is genetic fusion.
This enables the production of multifunctional enzymes that can be used for whole‐cell …

[HTML][HTML] Non-cleavable hinge enhances avidity and expansion of CAR-T cells for acute myeloid leukemia

MB Leick, H Silva, I Scarfò, R Larson, BD Choi… - Cancer cell, 2022 - cell.com
Chimeric antigen receptor (CAR) T cell therapy is effective in lymphoid malignancies, but
there has been limited data in myeloid cancers. Here, we start with a CD27-based CAR to …

A magnetic protein biocompass

S Qin, H Yin, C Yang, Y Dou, Z Liu, P Zhang, H Yu… - Nature materials, 2016 - nature.com
The notion that animals can detect the Earth's magnetic field was once ridiculed, but is now
well established. Yet the biological nature of such magnetosensing phenomenon remains …

Tuning the flexibility of glycine-serine linkers to allow rational design of multidomain proteins

M Van Rosmalen, M Krom, M Merkx - Biochemistry, 2017 - ACS Publications
Flexible polypeptide linkers composed of glycine and serine are important components of
engineered multidomain proteins. We have previously shown that the conformational …

Improving the reproducibility, accuracy, and stability of an electrochemical biosensor platform for point-of-care use

LC Chen, E Wang, CS Tai, YC Chiu, CW Li… - Biosensors and …, 2020 - Elsevier
Electrochemical biosensors possess numerous desirable qualities for target detection, such
as portability and ease of use, and are often considered for point-of-care (POC) …

[HTML][HTML] Design and biocatalytic applications of genetically fused multifunctional enzymes

DT Monterrey, I Ayuso-Fernández, I Oroz-Guinea… - Biotechnology …, 2022 - Elsevier
Fusion proteins, understood as those created by joining two or more genes that originally
encoded independent proteins, have numerous applications in biotechnology, from …

Protein mediated enzyme immobilization

AA Caparco, DR Dautel, JA Champion - Small, 2022 - Wiley Online Library
Enzyme immobilization is an essential technology for commercializing biocatalysis. It
imparts stability, recoverability, and other valuable features that improve the effectiveness of …

Spatial organization of multi-enzyme biocatalytic cascades

MB Quin, KK Wallin, G Zhang… - Organic & biomolecular …, 2017 - pubs.rsc.org
Industrial biocatalysis is an economically attractive option for the production of valuable
chemicals. Our repertoire of cheap building blocks and commodity target molecules is vastly …

Combinatorial metabolic pathway assembly approaches and toolkits for modular assembly

R Young, M Haines, M Storch, PS Freemont - Metabolic Engineering, 2021 - Elsevier
Synthetic Biology is a rapidly growing interdisciplinary field that is primarily built upon
foundational advances in molecular biology combined with engineering design principles …