The concept of engineering robust protein scaffolds for novel binding functions emerged 20
years ago, one decade after the advent of recombinant antibody technology. Early examples …

Eg5, the product of Kif11 gene, also known as kinesin spindle protein, is a motor protein
involved in the proper establishment of a bipolar mitotic spindle. Eg5 is one of the 45 …

Two mechanisms have been proposed for the function of motor proteins: The power stroke
and the Brownian ratchet. The former refers to generation of a large downhill free energy …

The dimeric motor protein kinesin-1 walks along microtubules by alternatingly hydrolyzing
ATP and moving two motor domains ('heads'). Nanometer-precision single-molecule studies …

Microtubule-based transport by the kinesin motors, powered by ATP hydrolysis, is essential
for a wide range of vital processes in eukaryotes. We obtained insight into this process by …

Kinesin-1, the founding member of the kinesin superfamily of proteins, is known to use only
a subset of microtubules for transport in living cells. This biased use of microtubules is …

The kinesin-3 motor KIF1A functions in neurons, where its fast and superprocessive motility
facilitates long-distance transport, but little is known about its force-generating properties …

Microtubules are cytoskeletal components involved in pivotal eukaryotic functions such as
cell division, ciliogenesis, and intracellular trafficking. They assemble from αβ-tubulin …

Liquid–liquid phase separation, occurs not only in bulk liquid, but also on surfaces. In
physiology, the nature and function of condensates on cellular structures remain …

To dissect the kinetics of structural transitions underlying the stepping cycle of kinesin-1 at
physiological ATP, we used interferometric scattering microscopy to track the position of gold …