Nucleic acids are excellent at recognizing complementary sequences through the formation
of Watson− Crick base pairs. Since the base pairing enables the highly selective …

Recent methodological advancements in microscopy and DNA sequencing-based methods
provide unprecedented new insights into the spatio-temporal relationships between …

Integrating biological imaging into early stages of the drug discovery process can provide
invaluable readouts of drug activity within complex disease settings, such as cancer …

Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining
diffusion and interaction/binding properties in biological and material sciences. An …

Metabolic reactions in living cells are limited by diffusion of reagents in the cytoplasm. Any
attempt to quantify the kinetics of biochemical reactions in the cytosol should be preceded by …

The location and number of neurotransmitter receptors are dynamically regulated at
postsynaptic sites. However, currently available methods for visualizing receptor trafficking …

We discuss a quantitative influence of macromolecular crowding on biological processes:
motion, bimolecular reactions, and gene expression in prokaryotic and eukaryotic cells. We …

Transcription factors (TFs) interact dynamically in vivo with chromatin binding sites. Here we
summarize and compare the four different techniques that are currently used to measure …

Fluorescence correlation spectroscopy (FCS) and fluorescence recovery after
photobleaching (FRAP) are widely used methods to determine diffusion coefficients …

Thermal motion of particles and molecules in liquids underlies many chemical and
biological processes. Liquids, especially in biology, are complex due to structure at multiple …