Forward and reverse genetic approaches for the analysis of vertebrate development in the zebrafish

ND Lawson, SA Wolfe - Developmental cell, 2011 - cell.com
The development of facile forward and reverse genetic approaches has propelled the
deconvolution of gene function in biology. While the origins of these techniques reside in the …

Retroviral insertional mutagenesis: past, present and future

AG Uren, J Kool, A Berns, M Van Lohuizen - Oncogene, 2005 - nature.com
Retroviral insertion mutagenesis screens in mice are powerful tools for efficient identification
of oncogenic mutations in an in vivo setting. Many oncogenes identified in these screens …

Gene transfer into genomes of human cells by the sleeping beauty transposon system

AM Geurts, Y Yang, KJ Clark, G Liu, Z Cui, AJ Dupuy… - Molecular Therapy, 2003 - cell.com
Abstract The Sleeping Beauty (SB) transposon system, derived from teleost fish sequences,
is extremely effective at delivering DNA to vertebrate genomes, including those of humans …

High-resolution genome-wide mapping of transposon integration in mammals

SR Yant, X Wu, Y Huang, B Garrison… - … and cellular biology, 2005 - Am Soc Microbiol
Abstract The Sleeping Beauty (SB) transposon is an emerging tool for transgenesis, gene
discovery, and therapeutic gene delivery in mammals. Here we studied 1,336 SB insertions …

In vivo protein trapping produces a functional expression codex of the vertebrate proteome

KJ Clark, D Balciunas, HM Pogoda, Y Ding… - Nature …, 2011 - nature.com
We describe a conditional in vivo protein-trap mutagenesis system that reveals
spatiotemporal protein expression dynamics and can be used to assess gene function in the …

Transposons as tools for functional genomics in vertebrate models

K Kawakami, DA Largaespada, Z Ivics - Trends in Genetics, 2017 - cell.com
Genetic tools and mutagenesis strategies based on transposable elements are currently
under development with a vision to link primary DNA sequence information to gene …

Nicotine response genetics in the zebrafish

AM Petzold, D Balciunas… - Proceedings of the …, 2009 - National Acad Sciences
Tobacco use is predicted to result in over 1 billion deaths worldwide by the end of the 21st
century. How genetic variation contributes to the observed differential predisposition in the …

Sleeping Beauty transposon‐mediated gene therapy for prolonged expression

PB Hackett, SC Ekker, DA Largaespada, RS McIvor - Advances in genetics, 2005 - Elsevier
Abstract The Sleeping Beauty (SB) transposon system represents a new vector for non‐viral
gene transfer that melds advantages of viruses and other forms of naked DNA transfer. The …

DNA transposons in vertebrate functional genomics

C Miskey, Z Izsvák, K Kawakami, Z Ivics - Cellular and molecular life …, 2005 - Springer
Genome sequences of many model organisms of developmental or agricultural importance
are becoming available. The tremendous amount of sequence data is fuelling the next …

Target-site preferences of Sleeping Beauty transposons

G Liu, AM Geurts, K Yae, AR Srinivasan… - Journal of molecular …, 2005 - Elsevier
The Sleeping Beauty (SB) transposon is a Tc1/mariner family transposon that has
applications in vertebrate animals for gene transfer, gene-tagging, and human gene therapy …