A SEVA-based, CRISPR-Cas3-assisted genome engineering approach for Pseudomonas with efficient vector curing
EM Lammens, DC Volke, K Schroven… - Microbiology …, 2023 - Am Soc Microbiol
The development of CRISPR-Cas-based engineering technologies has revolutionized the
microbial biotechnology field. Over the years, the Class II Type II CRISPR-Cas9 system has …
microbial biotechnology field. Over the years, the Class II Type II CRISPR-Cas9 system has …
An Orthogonal T7 Replisome for Continuous Hypermutation and Accelerated Evolution in E. coli
CS Diercks, P Sondermann, C Rong, DA Dik, TG Gillis… - bioRxiv, 2024 - biorxiv.org
Systems that perform continuous hypermutation of designated genes without compromising
the integrity of the host genome can dramatically accelerate the evolution of new or …
the integrity of the host genome can dramatically accelerate the evolution of new or …
Integration and Measurement for Building Synthetic Cells
AC Jackson-Smith - 2023 - search.proquest.com
Building a synthetic cell is a nebulous task, shrouded in the mysteries of life and what it is,
concealed in the uncertainty of what we know and don't know about biology, and entwined …
concealed in the uncertainty of what we know and don't know about biology, and entwined …
Systematic investigation of synthetic operon designs enables prediction and control of expression levels of multiple proteins
Controlling the expression levels of multiple recombinant proteins for optimal performance is
crucial for synthetic biosystems but remains difficult given the large number of DNA-encoded …
crucial for synthetic biosystems but remains difficult given the large number of DNA-encoded …