Biological systems are composed of heterogeneous populations of cells that
intercommunicate to form a functional living tissue. Biological function varies greatly across …

Organoids are 3D cell culture systems that mimic some of the structural and functional
characteristics of an organ. Organoid cultures provide the opportunity to study organ-level …

Mutations in genes involved in DNA methylation (DNAme; for example, TET2 and DNMT3A)
are frequently observed in hematological malignancies,–and clonal hematopoiesis …

Mammalian embryos sequentially differentiate into trophectoderm and an inner cell mass,
the latter of which differentiates into primitive endoderm and epiblast. Trophoblast stem (TS) …

Understanding gene regulation and function requires a genome-wide method capable of
capturing both gene expression levels and isoform diversity at the single-cell level. Short …

A central question in biology is whether variability between genetically identical cells
exposed to the same culture conditions is largely stochastic or deterministic. Using image …

Mouse embryonic stem (ES) cells grown in serum exhibit greater heterogeneity in
morphology and expression of pluripotency factors than ES cells cultured in defined medium …

A new level of chromosome organization, topologically associating domains (TADs), was
recently uncovered by chromosome conformation capture (3C) techniques. To explore TAD …

The use of two kinase inhibitors (2i) enables derivation of mouse embryonic stem cells
(ESCs) in the pluripotent ground state. Using whole-genome bisulfite sequencing (WGBS) …

Pluripotent stem cells (PSCs) are capable of dynamic interconversion between distinct
substates; however, the regulatory circuits specifying these states and enabling transitions …