Targeted proteomics for studying pathogenic bacteria

S Saleh, A Staes, S Deborggraeve, K Gevaert - Proteomics, 2019 - Wiley Online Library
Mass spectrometry‐based proteomics has been extensively used to map bacterial
proteomes, which has led to a better understanding of the molecular mechanisms …

Towards fully integrated liquid chromatography on a chip: evolution and evaluation

F Haghighi, Z Talebpour, AS Nezhad - TrAC Trends in Analytical Chemistry, 2018 - Elsevier
Miniaturization of liquid chromatography (LC) is at the leading edge of the research topics
and offers a wide range of applications in chemistry, biology, pharmaceutical, clinical …

Detection of Mycobacterium tuberculosis Peptides in the Exosomes of Patients with Active and Latent M. tuberculosis Infection Using MRM-MS

NA Kruh-Garcia, LM Wolfe, LH Chaisson… - PloS one, 2014 - journals.plos.org
The identification of easily measured, accurate diagnostic biomarkers for active tuberculosis
(TB) will have a significant impact on global TB control efforts. Because of the host and …

Second generation multiple reaction monitoring assays for enhanced detection of ultra-low abundance Mycobacterium tuberculosis peptides in human serum

C Mehaffy, KM Dobos, P Nahid, NA Kruh-Garcia - Clinical proteomics, 2017 - Springer
Abstract Background Mycobacterium tuberculosis (Mtb) is the causative agent of
Tuberculosis (TB), the number one cause of death due to an infectious disease. TB …

Chemical Proteomics Strategies for Analyzing Protein Lipidation Reveal the Bacterial O-Mycoloylome

N Banahene, TM Peters-Clarke, KJ Biegas… - Journal of the …, 2024 - ACS Publications
Protein lipidation dynamically controls protein localization and function within cellular
membranes. A unique form of protein O-fatty acylation in Corynebacterium, termed protein O …

Bidimensional Analyses of the Intra-and Extracellular Proteomes of Steroid Producer Mycobacteria

C Barreiro, AM Ibáñez - Microbial Steroids: Methods and Protocols, 2023 - Springer
The importance of the pathogenic mycobacteria has mainly focused the omic analyses on
different aspects of their clinical significance. However, those industrially relevant …

Mycoloyltransferases: A large and major family of enzymes shaping the cell envelope of Corynebacteriales

N Dautin, C de Sousa-d'Auria… - … et Biophysica Acta (BBA …, 2017 - Elsevier
Mycobacterium and Corynebacterium are important genera of the Corynebacteriales order,
the members of which are characterized by an atypical diderm cell envelope. Indeed the …

Review of liquid chromatography-mass spectrometry-based proteomic analyses of body fluids to diagnose infectious diseases

H Lee, SI Kim - International Journal of Molecular Sciences, 2022 - mdpi.com
Rapid and precise diagnostic methods are required to control emerging infectious diseases
effectively. Human body fluids are attractive clinical samples for discovering diagnostic …

Potential of high-affinity, slow off-rate modified aptamer reagents for Mycobacterium tuberculosis proteins as tools for infection models and diagnostic applications

TM Russell, LS Green, T Rice… - Journal of clinical …, 2017 - Am Soc Microbiol
Direct pathogen detection in blood to diagnose active tuberculosis (TB) has been difficult
due to low levels of circulating antigens or due to the lack of specific, high-affinity binding …

Virulence of Mycobacterium tuberculosis after Acquisition of Isoniazid Resistance: Individual Nature of katG Mutants and the Possible Role of AhpC

LM Nieto R, C Mehaffy, E Creissen, JL Troudt, A Troy… - PLoS …, 2016 - journals.plos.org
In the last decade, there were 10 million new tuberculosis cases per year globally. Around
9.5% of these cases were caused by isoniazid resistant (INHr) Mycobacterium tuberculosis …