Cellular stress and RNA splicing

G Biamonti, JF Caceres - Trends in biochemical sciences, 2009 - cell.com
In response to physical and chemical stresses that affect protein folding and, thus, the
execution of normal metabolic processes, cells activate gene-expression strategies aimed at …

Challenges and opportunities in the purification of recombinant tagged proteins

AS Pina, CR Lowe, ACA Roque - Biotechnology advances, 2014 - Elsevier
The purification of recombinant proteins by affinity chromatography is one of the most
efficient strategies due to the high recovery yields and purity achieved. However, this is …

Psip1/Ledgf p52 binds methylated histone H3K36 and splicing factors and contributes to the regulation of alternative splicing

MM Pradeepa, HG Sutherland, J Ule, GR Grimes… - PLoS …, 2012 - journals.plos.org
Increasing evidence suggests that chromatin modifications have important roles in
modulating constitutive or alternative splicing. Here we demonstrate that the PWWP domain …

Antagonistic role of hnRNP A1 and KSRP in the regulation of let-7a biogenesis

G Michlewski, JF Cáceres - Nature structural & molecular biology, 2010 - nature.com
The pluripotency-promoting proteins Lin28a and Lin28b act as post-transcriptional
repressors of let-7 miRNA biogenesis in undifferentiated embryonic stem cells. The levels of …

Posttranscriptional regulation of miRNAs harboring conserved terminal loops

G Michlewski, S Guil, CA Semple, JF Cáceres - Molecular cell, 2008 - cell.com
We recently found that hnRNP A1, a protein implicated in many aspects of RNA processing,
acts as an auxiliary factor for the Drosha-mediated processing of a microRNA precursor, pri …

The splicing factor SF2/ASF regulates translation initiation by enhancing phosphorylation of 4E-BP1

G Michlewski, JR Sanford, JF Cáceres - Molecular cell, 2008 - cell.com
Summary The SR protein SF2/ASF has been initially characterized as a splicing factor but
has also been shown to mediate postsplicing activities such as mRNA export and …

Cell-type targeted NF-kappaB inhibition for the treatment of inflammatory diseases

B Sehnert, H Burkhardt, S Dübel, RE Voll - Cells, 2020 - mdpi.com
Deregulated NF-k activation is not only involved in cancer but also contributes to the
pathogenesis of chronic inflammatory diseases like rheumatoid arthritis (RA) and multiple …

Reversible phosphorylation differentially affects nuclear and cytoplasmic functions of splicing factor 2/alternative splicing factor

JR Sanford, JD Ellis, D Cazalla… - Proceedings of the …, 2005 - National Acad Sciences
The Ser/Arg-rich (SR) proteins constitute a family of highly conserved nuclear
phosphoproteins that are involved in many steps of mRNA metabolism. Previously, we …

Genetic variation and RNA structure regulate microRNA biogenesis

N Fernandez, RA Cordiner, RS Young, N Hug… - Nature …, 2017 - nature.com
MiRNA biogenesis is highly regulated at the post-transcriptional level; however, the role of
sequence and secondary RNA structure in this process has not been extensively studied. A …

Identification of nuclear and cytoplasmic mRNA targets for the shuttling protein SF2/ASF

JR Sanford, P Coutinho, JA Hackett, X Wang… - PloS one, 2008 - journals.plos.org
The serine and arginine-rich protein family (SR proteins) are highly conserved regulators of
pre-mRNA splicing. SF2/ASF, a prototype member of the SR protein family, is a …