Advancements in CHO metabolomics: techniques, current state and evolving methodologies
R Singh, E Fatima, L Thakur, S Singh… - … in Bioengineering and …, 2024 - frontiersin.org
Background: Investigating the metabolic behaviour of different cellular phenotypes, ie,
good/bad grower and/or producer, in production culture is important to identify the key …
good/bad grower and/or producer, in production culture is important to identify the key …
Comprehensive stable-isotope tracing of glucose and amino acids identifies metabolic by-products and their sources in CHO cell culture
Mammalian cell culture processes are widely utilized for biotherapeutics production, disease
diagnostics, and biosensors, and hence, should be optimized to support robust cell growth …
diagnostics, and biosensors, and hence, should be optimized to support robust cell growth …
Amino acid degradation pathway inhibitory by‐products trigger apoptosis in CHO cells
Chinese hamster ovary (CHO) cells are widely used to produce complex
biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand …
biopharmaceuticals. Improving their productivity is necessary to fulfill the growing demand …
Metabolic Engineering of CHO Cells Towards Cysteine Prototrophy and Systems Analysis of The Ensuing Phenotype
L Greenfield, M Brantley, P Geoffroy, J Mitchell… - Metabolic …, 2024 - Elsevier
Chinese hamster ovary (CHO) cells require cysteine for growth and productivity in fed-batch
cultures. In intensified processes, supplementation of cysteine at high concentrations is a …
cultures. In intensified processes, supplementation of cysteine at high concentrations is a …
Strategies to improve CHO cell culture performance: Targeted deletion of amino acid catabolism and apoptosis genes paired with growth inhibitor supplementation
Chinese hamster ovary (CHO) cells are the predominant host of choice for recombinant
monoclonal antibody (mAb) expression. Recent advancements in gene editing technology …
monoclonal antibody (mAb) expression. Recent advancements in gene editing technology …
Multi-Scale Kinetics Modeling for Cell Culture Process with Metabolic State Transition
To advance the understanding of cellular metabolisms and control batch-to-batch variations
in cell culture processes, a multi-scale mechanistic model with a bottom-up and top-down …
in cell culture processes, a multi-scale mechanistic model with a bottom-up and top-down …
Harnessing Bacterial Periplasmic Binding Proteins as Biosensors for On-Line Process Analytical Technologies
L Wong - 2022 - search.proquest.com
Ligand-binding proteins have good to moderate binding affinity for various small molecules,
usually in the millimolar to micromolar range. Many industrially and clinically relevant …
usually in the millimolar to micromolar range. Many industrially and clinically relevant …
A Metabolomics Approach to Improving CHO Cell Productivity
G Yao - 2023 - search.proquest.com
Abstract Chinese Hamster Ovary (CHO) cells are widely used in the biopharmaceutical
industry to produce therapeutic recombinant proteins such as monoclonal antibodies for …
industry to produce therapeutic recombinant proteins such as monoclonal antibodies for …