Highlights•Kinetoplastids do not control transcription of individual protein-coding
genes.•mRNAs are made from polycistronic precursors by trans splicing and …

Kinetoplastids, including the human pathogens Trypanosoma brucei, Trypanosoma cruzi
and Leishmania, are the only known organisms that do not regulate the transcription of …

Transcription of protein-coding genes in trypanosomes is polycistronic and gene expression
is primarily regulated by post-transcriptional mechanisms. Sequence motifs in the …

The African trypanosome, Trypanosoma brucei, maintains an integral link between cell cycle
regulation and differentiation during its intricate life cycle. Whilst extensive changes in …

Developmental steps in the trypanosome life-cycle involve transition between replicative
and non-replicative forms specialised for survival in, and transmission between, mammalian …

Variant surface glycoprotein (VSG) coats bloodstream form Trypanosoma brucei parasites,
and monoallelic VSG expression underpins the antigenic variation necessary for …

We report a global quantitative phosphoproteomic study of bloodstream and procyclic form
Trypanosoma brucei using SILAC labeling of each lifecycle stage. Phosphopeptide …

Trans-splicing of leader sequences onto the 5′ ends of mRNAs is a widespread
phenomenon in protozoa, nematodes and some chordates. Using parallel sequencing we …

The protozoan parasite Trypanosoma brucei has a complex digenetic lifecycle between a
mammalian host and an insect vector, and adaption of its proteome between lifecycle stages …

Metabolomics coupled with heavy-atom isotope-labelled glucose has been used to probe
the metabolic pathways active in cultured bloodstream form trypomastigotes of …