Investigating freezing-induced acidity changes in citrate buffers

B Susrisweta, L Veselý, R Štůsek, A Hauptmann… - International journal of …, 2023 - Elsevier
Citrate buffers are commonly utilized in the field of biomolecule stabilization. We investigate
their applicability in the frozen state within a range of initial pHs (2.5 to 8.0) and …

A pH-responsive fluorometric and colorimetric system based on silicon quantum dots and 4-nitrophenol for urease activity detection

J Liu, J Zhang, Y Zhang, Y Wang, M Wang, Z Li… - Talanta, 2022 - Elsevier
In this paper, we proposed a dual-signal fluorometric and colorimetric system based on
silicon quantum dots (SiQDs) and 4-nitrophenol (4-NP) for pH and urease sensing. SiQDs …

Evaluation of the impact of esterases and lipases from the circulatory system against substrates of different lipophilicity

L Lam, MA Ilies - International Journal of Molecular Sciences, 2022 - mdpi.com
Esterases and lipases can process amphiphilic esters used as drugs and prodrugs and
impact their pharmacokinetics and biodistribution. These hydrolases can also process ester …

A high-throughput amenable colorimetric assay for enantioselective screening of nitrilase-producing microorganisms using pH sensitive indicators

A Banerjee, P Kaul, R Sharma… - Journal of …, 2003 - journals.sagepub.com
Based on the color change of an indicator due to the release of hydrogen ion from a nitrilase-
catalyzed reaction, a rapid colorimetric method was established for the enantioselective …

A pH-sensitive assay for galactosyltransferase

C Deng, RR Chen - Analytical biochemistry, 2004 - Elsevier
We report here a new pH-indicator-based assay for galactosyltransferase. The method is
simple and fast, requires no specialized equipment, labeled substrate, or other expensive …

Comparative analysis of high-throughput assays of family-1 plant glycosyltransferases

K McGraphery, W Schwab - International Journal of Molecular Sciences, 2020 - mdpi.com
The ability of glycosyltransferases (GTs) to reduce volatility, increase solubility, and thus
alter the bioavailability of small molecules through glycosylation has attracted immense …

A colorimetric assay for measuring phospholipase A2 degradation of phosphatidylcholine at physiological pH

JA Price III - Journal of Biochemical and Biophysical Methods, 2007 - Elsevier
Phospholipase A2 is an important enzyme in various pathologies. Although fluorescent
substrate assays for it have been recently developed, there is a need for an assay with …

Flexibility of Substrate Binding of Cytosine‐5′‐Monophosphate‐N‐Acetylneuraminate Synthetase (CMP‐Sialate Synthetase) from Neisseria meningitidis: An …

N He, D Yi, WD Fessner - Advanced Synthesis & Catalysis, 2011 - Wiley Online Library
We have successfully established a simple continuous colorimetric assay for the sensitive
and reliable quantification of cytosine‐5′‐monophosphate‐acetylneuraminate synthetase …

Making good's buffers good for freezing: the acidity changes and their elimination via mixing with sodium phosphate

L Veselý, B Susrisweta, D Heger - International Journal of Pharmaceutics, 2021 - Elsevier
Solutions of three Good's buffers (HEPES, MOPS, and MES), both pure and mixed with
sodium phosphate buffers (Na-P), are investigated in terms of the freezing-induced acidity …

Engineering of Candida antarctica lipase B for poly (ε-caprolactone) synthesis

CY Montanier, N Chabot, S Emond, D Guieysse… - European Polymer …, 2017 - Elsevier
Abstract Development of novel synthetic routes based on more eco-friendly processes, such
as enzyme-based catalysis, are in great demand to produce biopolymers. Among them …