Methods for physical characterization of phase-separated bodies and membrane-less organelles
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …
phase separation. This process enables compartmentalization of specific sets of …
Recent applications of light scattering measurement in the biological and biopharmaceutical sciences
AP Minton - Analytical biochemistry, 2016 - Elsevier
Measurement of the average intensity of excess scattering, termed static light scattering
(SLS), has become one of the most widely used techniques for the determination of molar …
(SLS), has become one of the most widely used techniques for the determination of molar …
Dynamics of proteins in solution
The dynamics of proteins in solution includes a variety of processes, such as backbone and
side-chain fluctuations, interdomain motions, as well as global rotational and translational …
side-chain fluctuations, interdomain motions, as well as global rotational and translational …
Multivalent ions and biomolecules: Attempting a comprehensive perspective
O Matsarskaia, F Roosen‐Runge… - ChemPhysChem, 2020 - Wiley Online Library
Ions are ubiquitous in nature. They play a key role for many biological processes on the
molecular scale, from molecular interactions, to mechanical properties, to folding, to self …
molecular scale, from molecular interactions, to mechanical properties, to folding, to self …
Understanding crystal nucleation mechanisms: where do we stand? General discussion
Kristen Fichthorn opened the discussion of the introductory lecture by James De Yoreo: I
noted that in your potential of mean force (PMF) plots for various particle orientations, …
noted that in your potential of mean force (PMF) plots for various particle orientations, …
[HTML][HTML] Ion-activated attractive patches as a mechanism for controlled protein interactions
The understanding of protein interactions to control phase and nucleation behavior of
protein solutions is an important challenge for soft matter, biological and medical research …
protein solutions is an important challenge for soft matter, biological and medical research …
[HTML][HTML] On the question of two-step nucleation in protein crystallization
We report a real-time study on protein crystallization in the presence of multivalent salts
using small angle X-ray scattering (SAXS) and optical microscopy, focusing particularly on …
using small angle X-ray scattering (SAXS) and optical microscopy, focusing particularly on …
Nonclassical nucleation
PG Vekilov - Crystallization via Nonclassical Pathways Volume 1 …, 2020 - ACS Publications
Nucleation determines the essential properties of a population of growing crystals including
the number of crystals, their size distribution, location, and polymorphs. In this chapter, we …
the number of crystals, their size distribution, location, and polymorphs. In this chapter, we …
Continuous capture of recombinant antibodies by ZnCl2 precipitation without polyethylene glycol
G Dutra, D Komuczki, A Jungbauer… - Engineering in life …, 2020 - Wiley Online Library
The capture of recombinant antibodies from cell culture broth is the first critical step of
downstream processing. We were able to develop a precipitation‐based method for the …
downstream processing. We were able to develop a precipitation‐based method for the …
Nonclassical nucleation pathways in protein crystallization
F Zhang - Journal of Physics: Condensed Matter, 2017 - iopscience.iop.org
Classical nucleation theory (CNT), which was established about 90 years ago, has been
very successful in many research fields, and continues to be the most commonly used theory …
very successful in many research fields, and continues to be the most commonly used theory …