Methods for physical characterization of phase-separated bodies and membrane-less organelles

DM Mitrea, B Chandra, MC Ferrolino, EB Gibbs… - Journal of molecular …, 2018 - Elsevier
Membrane-less organelles are cellular structures which arise through the phenomenon of
phase separation. This process enables compartmentalization of specific sets of …

Recent applications of light scattering measurement in the biological and biopharmaceutical sciences

AP Minton - Analytical biochemistry, 2016 - Elsevier
Measurement of the average intensity of excess scattering, termed static light scattering
(SLS), has become one of the most widely used techniques for the determination of molar …

Dynamics of proteins in solution

M Grimaldo, F Roosen-Runge, F Zhang… - Quarterly Reviews of …, 2019 - cambridge.org
The dynamics of proteins in solution includes a variety of processes, such as backbone and
side-chain fluctuations, interdomain motions, as well as global rotational and translational …

Multivalent ions and biomolecules: Attempting a comprehensive perspective

O Matsarskaia, F Roosen‐Runge… - ChemPhysChem, 2020 - Wiley Online Library
Ions are ubiquitous in nature. They play a key role for many biological processes on the
molecular scale, from molecular interactions, to mechanical properties, to folding, to self …

Understanding crystal nucleation mechanisms: where do we stand? General discussion

MW Anderson, M Bennett, R Cedeno, H Cölfen… - Faraday …, 2022 - pubs.rsc.org
Kristen Fichthorn opened the discussion of the introductory lecture by James De Yoreo: I
noted that in your potential of mean force (PMF) plots for various particle orientations, …

[HTML][HTML] Ion-activated attractive patches as a mechanism for controlled protein interactions

F Roosen-Runge, F Zhang, F Schreiber, R Roth - Scientific reports, 2014 - nature.com
The understanding of protein interactions to control phase and nucleation behavior of
protein solutions is an important challenge for soft matter, biological and medical research …

[HTML][HTML] On the question of two-step nucleation in protein crystallization

A Sauter, F Roosen-Runge, F Zhang, G Lotze… - Faraday …, 2015 - pubs.rsc.org
We report a real-time study on protein crystallization in the presence of multivalent salts
using small angle X-ray scattering (SAXS) and optical microscopy, focusing particularly on …

Nonclassical nucleation

PG Vekilov - Crystallization via Nonclassical Pathways Volume 1 …, 2020 - ACS Publications
Nucleation determines the essential properties of a population of growing crystals including
the number of crystals, their size distribution, location, and polymorphs. In this chapter, we …

Continuous capture of recombinant antibodies by ZnCl2 precipitation without polyethylene glycol

G Dutra, D Komuczki, A Jungbauer… - Engineering in life …, 2020 - Wiley Online Library
The capture of recombinant antibodies from cell culture broth is the first critical step of
downstream processing. We were able to develop a precipitation‐based method for the …

Nonclassical nucleation pathways in protein crystallization

F Zhang - Journal of Physics: Condensed Matter, 2017 - iopscience.iop.org
Classical nucleation theory (CNT), which was established about 90 years ago, has been
very successful in many research fields, and continues to be the most commonly used theory …