The continual demand for specialized molecular cloning techniques that suit a broad range
of applications has driven the development of many different cloning strategies. One method …

In 1917, a food chemist named James Currie made a promising discovery: any strain of the
filamentous mould Aspergillus niger would produce high concentrations of citric acid when …

The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial
enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is …

Fungi are an understudied resource possessing huge potential for developing products that
can greatly improve human well-being. In the current paper, we highlight some important …

Aspergillus niger has become one of the most important hosts for food enzyme production
due to its unique food safety characteristics and excellent protein secretion systems. A series …

Besides its use for efficient production of recombinant proteins the methylotrophic yeast
Pichia pastoris (syn. Komagataella spp.) has been increasingly employed as a platform to …

Filamentous fungi play an important role in human health and industrial/agricultural
production. With the increasing number of full genomes available for fungal species, the …

As redesigning organisms using engineering principles is one of the purposes of synthetic
biology (SynBio), the standardization of experimental methods and DNA parts is becoming …

Aspergillus niger was engineered using a gene responsible for citric acid transport, which
has a significant impact on citric acid secretion when overexpressed. The transport gene …

The CRISPR/Cas9 system is a revolutionary genome editing tool. However, in eukaryotes,
search and optimization of a suitable promoter for guide RNA expression is a significant …