Recent advances in enzyme extraction strategies: A comprehensive review

SS Nadar, RG Pawar, VK Rathod - International journal of biological …, 2017 - Elsevier
The increasing interest of industrial enzymes demands for development of new downstream
strategies for maximizing enzyme recovery. The significant efforts have been focused on the …

Affinity‐Controlled Partitioning of Biomolecules at Aqueous Interfaces and Their Bioanalytic Applications

Y Cao, Y Chao, HC Shum - Advanced Materials, 2024 - Wiley Online Library
All‐aqueous phase separation systems play essential roles in bioanalytical and biochemical
applications. Compared to conventional oil and organic solvent‐based systems, these …

Virus isoelectric point determination using single-particle chemical force microscopy

X Mi, EK Bromley, PU Joshi, F Long, CL Heldt - Langmuir, 2019 - ACS Publications
Virus colloidal behavior is governed by the interaction of the viral surface and the
surrounding environment. One method to characterize the virus surface charge is the …

The downstream bioprocess toolbox for therapeutic viral vectors

R Kilgore, A Minzoni, S Shastry, W Smith… - … of Chromatography A, 2023 - Elsevier
Viral vectors are poised to acquire a prominent position in modern medicine and
biotechnology owing to their role as delivery agents for gene therapies, oncolytic agents …

Experimental and computational surface hydrophobicity analysis of a non-enveloped virus and proteins

CL Heldt, A Zahid, KS Vijayaragavan, X Mi - Colloids and Surfaces B …, 2017 - Elsevier
The physical characteristics of viruses needs to be understood in order to manipulate the
interaction of viruses with host cells, as well as to create specific molecular recognition …

Phase diagrams of aqueous two-phase systems formed by polyethylene glycol+ ammonium sulfate+ water: equilibrium data and thermodynamic modeling

GF Murari, JA Penido, PAL Machado, LR de Lemos… - Fluid Phase …, 2015 - Elsevier
Phase diagrams of aqueous two-phase systems (ATPS) composed of polyethylene glycol
(PEG) of different molar mass (400 or 10,000 or 35,000) g mol− 1+ ammonium sulfate+ …

Continuous purification of an enveloped and non-enveloped viral particle using an aqueous two-phase system

DG Turpeinen, PU Joshi, SA Kriz, S Kaur… - Separation and …, 2021 - Elsevier
Meeting the increasing demand for vaccines throughout the world is key to decrease the
spread of infectious diseases. The switch to a fully continuous vaccine manufacturing …

Separation and purification of enveloped and non-enveloped viruses from water samples using an aqueous two-phase system

X Hao, J Chen, M Xu, H Zheng, X Li, M Wang, T Liu - Process Biochemistry, 2022 - Elsevier
In this study, a new strategy for enriching viruses in water was developed to improve the
detection of viruses in environmental samples. The virus was enriched from water by …

Recovery and primary purification of bacteriophage M13 using aqueous two‐phase systems

A González‐Mora, F Ruiz‐Ruiz… - Journal of Chemical …, 2017 - Wiley Online Library
BACKGROUND Bacteriophage M13 is an Escherichia coli‐specific non‐lytic filamentous
virus commonly used in applications ranging from antibody screening and nanomaterial …

Tie line framework to optimize non-enveloped virus recovery in aqueous two-phase systems

PU Joshi, DG Turpeinen, M Weiss… - … of Chromatography B, 2019 - Elsevier
Viral particle purification is a challenge due to the complexity of the broth, the particle size,
and the need to maintain virus activity. Aqueous two-phase systems (ATPSs) are a viable …