A localized nucleolar DNA damage response facilitates recruitment of the homology-directed repair machinery independent of cell cycle stage
M van Sluis, B McStay - Genes & development, 2015 - genesdev.cshlp.org
DNA double-strand breaks (DSBs) are repaired by two main pathways: nonhomologous end-
joining and homologous recombination (HR). Repair pathway choice is thought to be …
joining and homologous recombination (HR). Repair pathway choice is thought to be …
Homing endonucleases: from basics to therapeutic applications
MJ Marcaida, IG Muñoz, FJ Blanco, J Prieto… - Cellular and Molecular …, 2010 - Springer
Homing endonucleases (HE) are double-stranded DNAses that target large recognition sites
(12–40 bp). HE-encoding sequences are usually embedded in either introns or inteins. Their …
(12–40 bp). HE-encoding sequences are usually embedded in either introns or inteins. Their …
How Can One Metal Power Nucleic Acid Phosphodiester Bond Cleavage by a Nuclease? Multiscale Computational Studies Highlight a Diverse Mechanistic …
DJ Nikkel, R Kaur, SD Wetmore - The Journal of Physical …, 2024 - ACS Publications
Despite the remarkable resistance of the nucleic acid phosphodiester backbone to
degradation affording genetic stability, the P–O bond must be broken during DNA repair and …
degradation affording genetic stability, the P–O bond must be broken during DNA repair and …
Linear nicking endonuclease-mediated strand-displacement DNA amplification
A Joneja, X Huang - Analytical biochemistry, 2011 - Elsevier
We describe a method for linear isothermal DNA amplification using nicking endonuclease-
mediated strand displacement by a DNA polymerase. The nicking of one strand of a DNA …
mediated strand displacement by a DNA polymerase. The nicking of one strand of a DNA …
Elucidation of the catalytic mechanism of a single-metal dependent homing endonuclease using QM and QM/MM approaches: the case study of I-Ppo I
R Kaur, A Frederickson, SD Wetmore - Physical Chemistry Chemical …, 2024 - pubs.rsc.org
Homing endonucleases (HEs) are highly specific DNA cleaving enzymes, with I-PpoI having
been suggested to use a single metal to accelerate phosphodiester bond cleavage …
been suggested to use a single metal to accelerate phosphodiester bond cleavage …
Pushing the limits of what is achievable in protein–DNA docking: benchmarking HADDOCK's performance
M van Dijk, AMJJ Bonvin - Nucleic acids research, 2010 - academic.oup.com
The intrinsic flexibility of DNA and the difficulty of identifying its interaction surface have long
been challenges that prevented the development of efficient protein–DNA docking methods …
been challenges that prevented the development of efficient protein–DNA docking methods …
Successful Targeting and Disruption of an Integrated Reporter Lentivirus Using the Engineered Homing Endonuclease Y2 I-AniI
M Aubert, BY Ryu, L Banks, DJ Rawlings… - PloS one, 2011 - journals.plos.org
Current antiviral therapy does not cure HIV-infected individuals because the virus
establishes lifelong latent infection within long-lived memory T cells as integrated HIV …
establishes lifelong latent infection within long-lived memory T cells as integrated HIV …
Comprehensive computational design of mCreI homing endonuclease cleavage specificity for genome engineering
UY Ulge, DA Baker, RJ Monnat Jr - Nucleic acids research, 2011 - academic.oup.com
Homing endonucleases (HEs) cleave long (∼ 20 bp) DNA target sites with high site
specificity to catalyze the lateral transfer of parasitic DNA elements. In order to determine …
specificity to catalyze the lateral transfer of parasitic DNA elements. In order to determine …
Targeted approaches for gene therapy and the emergence of engineered meganucleases
R Galetto, P Duchateau, F Pâques - Expert Opinion on Biological …, 2009 - Taylor & Francis
Background: In spite of significant advances in gene transfer strategies in the field of gene
therapy, there is a strong emphasis on the development of alternative methods, providing …
therapy, there is a strong emphasis on the development of alternative methods, providing …
Homing endonucleases catalyze double‐stranded DNA breaks and somatic transgene excision in Aedes aegypti
BE Traver, MAE Anderson… - Insect molecular …, 2009 - Wiley Online Library
Aedes aegypti is a major vector of arthropod‐borne viruses such as yellow fever virus and
dengue viruses. Efforts to discern the function of genes involved in important behaviours …
dengue viruses. Efforts to discern the function of genes involved in important behaviours …