Migratory cells are polarized with protrusive fronts and contractile rears. This spatial
organization necessitates long-range coordination of the signals that organize protrusions …

Ras has been extensively studied as a promoter of cell proliferation, whereas few studies
have explored its role in migration. To investigate the direct and immediate effects of Ras …

In response to different immune challenges, immune cells migrate to specific sites in the
body, where they perform their functions such as defense against infection, inflammation …

In this article, we provide detailed protocols on using optogenetic dimerizers to acutely
perturb activities of guanine nucleotide exchange factors (GEFs) specific to Ras, Rac or Rho …

Phosphoinositides broadly impact membrane dynamics, signal transduction and cellular
physiology. The orchestration of signaling complexity by this seemingly simple metabolic …

The social amoeba Dictyostelium discoideum provides an excellent model for research
across a broad range of disciplines within biology. The organism diverged from the plant …

Symmetry breaking, polarity establishment, and spontaneous cell protrusion formation are
fundamental but poorly explained cell behaviors. Here, we demonstrate that a biochemical …

Studies in the model systems, Dictyostelium amoebae and HL-60 neutrophils, have shown
that local Ras activity directly regulates cell motility or polarity. Localized Ras activation on …

Functions of Ras oncogenes and their downstream effectors are typically associated with
cell proliferation and growth control while their role in immune cell migration has been …

Ras signaling is typically associated with cell growth, but not direct regulation of motility or
polarity. By optogenetically targeting different nodes in the Ras/PI3K/Akt network in …