Stem-cell-based embryo models for fundamental research and translation

J Fu, A Warmflash, MP Lutolf - Nature materials, 2021 - nature.com
Despite its importance, understanding the early phases of human development has been
limited by availability of human samples. The recent emergence of stem-cell-derived embryo …

[HTML][HTML] Mouse gastrulation: Coordination of tissue patterning, specification and diversification of cell fate

ES Bardot, AK Hadjantonakis - Mechanisms of development, 2020 - Elsevier
During mouse embryonic development a mass of pluripotent epiblast tissue is transformed
during gastrulation to generate the three definitive germ layers: endoderm, mesoderm, and …

Anteroposterior polarity and elongation in the absence of extra-embryonic tissues and of spatially localised signalling in gastruloids: mammalian embryonic organoids

DA Turner, M Girgin, L Alonso-Crisostomo… - …, 2017 - journals.biologists.com
The establishment of the anteroposterior (AP) axis is a crucial step during animal embryo
development. In mammals, genetic studies have shown that this process relies on signals …

NANOG and CDX2 pattern distinct subtypes of human mesoderm during exit from pluripotency

S Mendjan, VL Mascetti, D Ortmann, M Ortiz… - Cell stem cell, 2014 - cell.com
Mesoderm is induced at the primitive streak (PS) and patterns subsequently into
mesodermal subtypes and organ precursors. It is unclear whether mesoderm induction …

[HTML][HTML] Gastruloids generated without exogenous Wnt activation develop anterior neural tissues

MU Girgin, N Broguiere, L Mattolini, MP Lutolf - Stem cell reports, 2021 - cell.com
When stimulated with a pulse from an exogenous WNT pathway activator, small aggregates
of mouse embryonic stem cells (ESCs) can undergo embryo-like axial morphogenesis and …

Gastruloids: Pluripotent stem cell models of mammalian gastrulation and embryo engineering

AM Arias, Y Marikawa, N Moris - Developmental biology, 2022 - Elsevier
Gastrulation is a fundamental and critical process of animal development whereby the mass
of cells that results from the proliferation of the zygote transforms itself into a recognizable …

β-Catenin regulates primitive streak induction through collaborative interactions with SMAD2/SMAD3 and OCT4

NS Funa, KA Schachter, M Lerdrup, J Ekberg, K Hess… - Cell stem cell, 2015 - cell.com
Summary Canonical Wnt and Nodal signaling are both required for induction of the primitive
streak (PS), which guides organization of the early embryo. The Wnt effector β-catenin is …

Endogenous WNT signals mediate BMP-induced and spontaneous differentiation of epiblast stem cells and human embryonic stem cells

D Kurek, A Neagu, M Tastemel, N Tüysüz, J Lehmann… - Stem cell reports, 2015 - cell.com
Therapeutic application of human embryonic stem cells (hESCs) requires precise control
over their differentiation. However, spontaneous differentiation is prevalent, and growth …

Stem-cell-based human and mouse embryo models

M Bao, J Cornwall-Scoones… - Current opinion in genetics …, 2022 - Elsevier
Synthetic embryology aims to develop embryo-like structures from stem cells to provide new
insight into early stages of mammalian development. Recent advances in synthetic …

The dynamics of morphogenesis in the early mouse embryo

JA Rivera-Pérez… - Cold Spring Harbor …, 2015 - cshperspectives.cshlp.org
Over the past two decades, our understanding of mouse development from implantation to
gastrulation has grown exponentially with an upsurge of genetic, molecular, cellular, and …