Isolation of extracellular vesicles: general methodologies and latest trends

MY Konoshenko, EA Lekchnov… - BioMed research …, 2018 - Wiley Online Library
Background. Extracellular vesicles (EVs) play an essential role in the communication
between cells and transport of diagnostically significant molecules. A wide diversity of …

Technical challenges of working with extracellular vesicles

MI Ramirez, MG Amorim, C Gadelha, I Milic, JA Welsh… - Nanoscale, 2018 - pubs.rsc.org
Extracellular Vesicles (EVs) are gaining interest as central players in liquid biopsies, with
potential applications in diagnosis, prognosis and therapeutic guidance in most pathological …

Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles

U Erdbrügger, CJ Blijdorp, IV Bijnsdorp… - Journal of …, 2021 - Wiley Online Library
Urine is commonly used for clinical diagnosis and biomedical research. The discovery of
extracellular vesicles (EV) in urine opened a new fast‐growing scientific field. In the last …

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the …

C Théry, KW Witwer, E Aikawa… - Journal of …, 2018 - Wiley Online Library
The last decade has seen a sharp increase in the number of scientific publications
describing physiological and pathological functions of extracellular vesicles (EVs), a …

A review on comparative studies addressing exosome isolation methods from body fluids

TS Martins, M Vaz, AG Henriques - Analytical and Bioanalytical Chemistry, 2023 - Springer
Exosomes emerged as valuable sources of disease biomarkers and new therapeutic tools.
However, extracellular vesicles isolation with exosome-like characteristics from certain …

Isolation and characterization of urinary extracellular vesicles: implications for biomarker discovery

ML Merchant, IM Rood, JKJ Deegens… - Nature Reviews …, 2017 - nature.com
Urine is a valuable diagnostic medium and, with the discovery of urinary extracellular
vesicles, is viewed as a dynamic bioactive fluid. Extracellular vesicles are lipid-enclosed …

Standardization of sample collection, isolation and analysis methods in extracellular vesicle research

KW Witwer, EI Buzás, LT Bemis, A Bora… - Journal of …, 2013 - Taylor & Francis
The emergence of publications on extracellular RNA (exRNA) and extracellular vesicles
(EV) has highlighted the potential of these molecules and vehicles as biomarkers of disease …

Extracellular vesicles as a source of prostate cancer biomarkers in liquid biopsies: a decade of research

M Ramirez-Garrastacho, C Bajo-Santos, A Line… - British journal of …, 2022 - nature.com
Prostate cancer is a global cancer burden and considerable effort has been made through
the years to identify biomarkers for the disease. Approximately a decade ago, the potential of …

Unravelling the proteomic landscape of extracellular vesicles in prostate cancer by density-based fractionation of urine

B Dhondt, E Geeurickx, J Tulkens… - Journal of …, 2020 - Taylor & Francis
Extracellular vesicles (EV) are increasingly being recognized as important vehicles of
intercellular communication and promising diagnostic and prognostic biomarkers in cancer …

Improved isolation strategies to increase the yield and purity of human urinary exosomes for biomarker discovery

AH Gheinani, M Vögeli, U Baumgartner, E Vassella… - Scientific reports, 2018 - nature.com
Circulating miRNAs are detected in extracellular space and body fluids such as urine.
Circulating RNAs can be packaged in secreted urinary extracellular vesicles (uEVs) and …