Genetic diversity is indispensable for plant breeding to improve crops

S Swarup, EJ Cargill, K Crosby, L Flagel… - Crop …, 2021 - Wiley Online Library
Plant breeders face multiple global challenges that affect food security, productivity,
accessibility, and nutritional quality. One major challenge for plant breeders is developing …

Progress and prospects in plant genome editing

K Yin, C Gao, JL Qiu - Nature plants, 2017 - nature.com
The emergence of sequence-specific nucleases that enable genome editing is
revolutionizing basic and applied biology. Since the introduction of CRISPR–Cas9, genome …

[HTML][HTML] Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

S Svitashev, C Schwartz, B Lenderts, JK Young… - Nature …, 2016 - nature.com
Targeted DNA double-strand breaks have been shown to significantly increase the
frequency and precision of genome editing. In the past two decades, several double-strand …

[HTML][HTML] Generation of high-amylose rice through CRISPR/Cas9-mediated targeted mutagenesis of starch branching enzymes

Y Sun, G Jiao, Z Liu, X Zhang, J Li, X Guo… - Frontiers in plant …, 2017 - frontiersin.org
Cereals high in amylose content (AC) and resistant starch (RS) offer potential health
benefits. Previous studies using chemical mutagenesis or RNA interference have …

Targeted mutagenesis, precise gene editing, and site-specific gene insertion in maize using Cas9 and guide RNA

S Svitashev, JK Young, C Schwartz, H Gao… - Plant …, 2015 - academic.oup.com
Targeted mutagenesis, editing of endogenous maize (Zea mays) genes, and site-specific
insertion of a trait gene using clustered regularly interspaced short palindromic repeats …

[HTML][HTML] Gene editing and crop improvement using CRISPR-Cas9 system

L Arora, A Narula - Frontiers in plant science, 2017 - frontiersin.org
Advancements in Genome editing technologies have revolutionized the fields of functional
genomics and crop improvement. CRISPR/Cas9 (clustered regularly interspaced short …

A CRISPR/Cas9 toolbox for multiplexed plant genome editing and transcriptional regulation

LG Lowder, D Zhang, NJ Baltes, JW Paul III… - Plant …, 2015 - academic.oup.com
The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

[HTML][HTML] CRISPR/Cas9 platforms for genome editing in plants: developments and applications

X Ma, Q Zhu, Y Chen, YG Liu - Molecular plant, 2016 - cell.com
The clustered regularly interspaced short palindromic repeat (CRISPR)-associated protein9
(Cas9) genome editing system (CRISPR/Cas9) is adapted from the prokaryotic type II …

CRISPR crops: plant genome editing toward disease resistance

T Langner, S Kamoun, K Belhaj - Annual review of …, 2018 - annualreviews.org
Genome editing by sequence-specific nucleases (SSNs) has revolutionized biology by
enabling targeted modifications of genomes. Although routine plant genome editing …

Both CRISPR/Cas‐based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana

F Fauser, S Schiml, H Puchta - The Plant Journal, 2014 - Wiley Online Library
Engineered nucleases can be used to induce site‐specific double‐strand breaks (DSB s) in
plant genomes. Thus, homologous recombination (HR) can be enhanced and targeted …