Directed evolution: methodologies and applications
Directed evolution aims to expedite the natural evolution process of biological molecules
and systems in a test tube through iterative rounds of gene diversifications and library …
and systems in a test tube through iterative rounds of gene diversifications and library …
Quality control in the endoplasmic reticulum
L Ellgaard, A Helenius - Nature reviews Molecular cell biology, 2003 - nature.com
The endoplasmic reticulum (ER) has a quality-control system for'proof-reading'newly
synthesized proteins, so that only native conformers reach their final destinations. Non …
synthesized proteins, so that only native conformers reach their final destinations. Non …
Selecting and screening recombinant antibody libraries
HR Hoogenboom - Nature biotechnology, 2005 - nature.com
During the past decade several display methods and other library screening techniques
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …
have been developed for isolating monoclonal antibodies (mAbs) from large collections of …
Applications of yeast surface display for protein engineering
GM Cherf, JR Cochran - Yeast Surface Display: Methods, Protocols, and …, 2015 - Springer
The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae
via genetic fusion to an abundant cell wall protein, a technology known as yeast surface …
via genetic fusion to an abundant cell wall protein, a technology known as yeast surface …
Engineering novel binding proteins from nonimmunoglobulin domains
HK Binz, P Amstutz, A Plückthun - Nature biotechnology, 2005 - nature.com
Not all adaptive immune systems use the immunoglobulin fold as the basis for specific
recognition molecules: sea lampreys, for example, have evolved an adaptive immune …
recognition molecules: sea lampreys, for example, have evolved an adaptive immune …
Directed evolution of human T-cell receptors with picomolar affinities by phage display
Y Li, R Moysey, PE Molloy, AL Vuidepot, T Mahon… - Nature …, 2005 - nature.com
Peptides derived from almost all proteins, including disease-associated proteins, can be
presented on the cell surface as peptide–human leukocyte antigen (pHLA) complexes. T …
presented on the cell surface as peptide–human leukocyte antigen (pHLA) complexes. T …
Flow-cytometric isolation of human antibodies from a nonimmune Saccharomyces cerevisiae surface display library
MJ Feldhaus, RW Siegel, LK Opresko… - Nature …, 2003 - nature.com
A nonimmune library of 109 human antibody scFv fragments has been cloned and
expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by …
expressed on the surface of yeast, and nanomolar-affinity scFvs routinely obtained by …
Development of a humanized monoclonal antibody with therapeutic potential against West Nile virus
T Oliphant, M Engle, GE Nybakken, C Doane… - Nature medicine, 2005 - nature.com
Abstract Neutralization of West Nile virus (WNV) in vivo correlates with the development of
an antibody response against the viral envelope (E) protein. Using random mutagenesis …
an antibody response against the viral envelope (E) protein. Using random mutagenesis …
[25] Yeast surface display for directed evolution of protein expression, affinity, and stability
ET Boder, KD Wittrup - Methods in enzymology, 2000 - Elsevier
The described protocols enable thorough screening of polypeptide libraries with high
confidence in the isolation of improved clones. It should be emphasized that the protocols …
confidence in the isolation of improved clones. It should be emphasized that the protocols …
Yeast cell-surface display—applications of molecular display
A Kondo, M Ueda - Applied microbiology and biotechnology, 2004 - Springer
In a cell-surface engineering system established using the yeast Saccharomyces cerevisiae,
novel, so-called arming yeasts are constructed that are armed with biocatalysts in the form of …
novel, so-called arming yeasts are constructed that are armed with biocatalysts in the form of …