Long-read sequencing–a powerful tool in viral transcriptome research

Z Boldogkői, N Moldován, Z Balázs, M Snyder… - Trends in …, 2019 - cell.com
Long-read sequencing (LRS) has become increasingly popular due to its strengths in de
novo assembly and in resolving complex DNA regions as well as in determining full-length …

Dynamic nanopore long-read sequencing analysis of HIV-1 splicing events during the early steps of infection

N Nguyen Quang, S Goudey, E Ségéral, A Mohammad… - Retrovirology, 2020 - Springer
Background Alternative splicing is a key step in Human Immunodeficiency Virus type 1 (HIV-
1) replication that is tightly regulated both temporally and spatially. More than 50 different …

Multiple long-read sequencing survey of herpes simplex virus dynamic transcriptome

D Tombácz, N Moldován, Z Balázs, G Gulyás… - Frontiers in …, 2019 - frontiersin.org
Long-read sequencing (LRS) has become increasingly important in RNA research due to its
strength in resolving complex transcriptomic architectures. In this regard, currently two LRS …

In-depth temporal transcriptome profiling of monkeypox and host cells using nanopore sequencing

B Kakuk, Á Dörmő, Z Csabai, G Kemenesi, J Holoubek… - Scientific Data, 2023 - nature.com
The recent human Monkeypox outbreak underlined the importance of studying basic biology
of orthopoxviruses. However, the transcriptome of its causative agent has not been …

NanoPipe—a web server for nanopore MinION sequencing data analysis

V Shabardina, T Kischka, F Manske… - …, 2019 - academic.oup.com
Background The fast-moving progress of the third-generation long-read sequencing
technologies will soon bring the biological and medical sciences to a new era of research …

Long-read assays shed new light on the transcriptome complexity of a viral pathogen

D Tombácz, I Prazsák, Z Csabai, N Moldován… - Scientific Reports, 2020 - nature.com
Abstract Characterization of global transcriptomes using conventional short-read
sequencing is challenging due to the insensitivity of these platforms to transcripts isoforms …

Vaccinia virus Tiantan strain blocks host antiviral innate immunity and programmed cell death by disrupting gene expression

C Wu, Z Zhang, Z Li, R Li, S Huo, H Li, R Lu… - Biosafety and …, 2024 - mednexus.org
The vaccinia virus Tiantan (VTT) is widely utilized as a smallpox vaccine in China and holds
significant importance in the prevention of diseases stemming from poxvirus infections …

Combined nanopore and single-molecule real-time sequencing survey of human betaherpesvirus 5 transcriptome

B Kakuk, D Tombácz, Z Balázs, N Moldován… - Scientific Reports, 2021 - nature.com
Abstract Long-read sequencing (LRS), a powerful novel approach, is able to read full-length
transcripts and confers a major advantage over the earlier gold standard short-read …

Time-course profiling of bovine alphaherpesvirus 1.1 transcriptome using multiplatform sequencing

N Moldován, G Torma, G Gulyás, Á Hornyák… - Scientific Reports, 2020 - nature.com
Long-read sequencing (LRS) has become a standard approach for transcriptome analysis in
recent years. Bovine alphaherpesvirus 1 (BoHV-1) is an important pathogen of cattle …

Using direct RNA nanopore sequencing to deconvolute viral transcriptomes

DP Depledge, AC Wilson - Current protocols in microbiology, 2020 - Wiley Online Library
The genomes of DNA viruses encode deceptively complex transcriptomes evolved to
maximize coding potential within the confines of a relatively small genome. Defining the full …