CRISPR-based technologies represent a major breakthrough in biomedical science as they
offer a powerful platform for unbiased screening and functional genomics in various fields …

Ferroptosis is widely involved in degenerative diseases in various tissues including kidney,
liver and brain, and is a targetable vulnerability in multiple primary and therapy-resistant …

Here we provide a detailed tutorial on CRISPR in vivo screening. Using the mouse as the
model organism, we introduce a range of CRISPR tools and applications, delineate general …

Clear-cell carcinomas (CCCs) are a histological group of highly aggressive malignancies
commonly originating in the kidney and ovary. CCCs are distinguished by aberrant lipid and …

Multimodal single-cell assays provide high-resolution snapshots of complex cell
populations, but are mostly limited to transcriptome plus an additional modality. Here, we …

Background Many functional analysis tools have been developed to extract functional and
mechanistic insight from bulk transcriptome data. With the advent of single-cell RNA …

Cas12a RNA-guided endonucleases are promising tools for multiplexed genetic
perturbations because they can process multiple guide RNAs expressed as a single …

Adoptive transfer of genetically modified immune cells holds great promise for cancer
immunotherapy. CRISPR knockin targeting can improve cell therapies, but more high …

Examining host–pathogen interactions in animals can capture aspects of infection that are
obscured in cell culture. Using CRISPR-based screens, we functionally profile the entire …

CRISPR-based genetic screening has revolutionized cancer drug target discovery, yet
reliable, multiplex gene editing to reveal synergies between gene targets remains a major …