Exploiting and engineering hemoproteins for abiological carbene and nitrene transfer reactions
Highlights•Engineered hemoproteins expand the biocatalytic repertoire.•Different
hemoprotein scaffolds offer diverse activities and selectivities.•New enzymes enable …
hemoprotein scaffolds offer diverse activities and selectivities.•New enzymes enable …
Cytochromes P450 for natural product biosynthesis in Streptomyces: sequence, structure, and function
Covering: up to January 2017 Cytochrome P450 enzymes (P450s) are some of the most
exquisite and versatile biocatalysts found in nature. In addition to their well-known roles in …
exquisite and versatile biocatalysts found in nature. In addition to their well-known roles in …
[HTML][HTML] Diversity and evolution of the P450 family in arthropods
The P450 family (CYP genes) of arthropods encodes diverse enzymes involved in the
metabolism of foreign compounds and in essential endocrine or ecophysiological functions …
metabolism of foreign compounds and in essential endocrine or ecophysiological functions …
An artificial metalloenzyme with the kinetics of native enzymes
P Dydio, HM Key, A Nazarenko, JYE Rha… - Science, 2016 - science.org
Natural enzymes contain highly evolved active sites that lead to fast rates and high
selectivities. Although artificial metalloenzymes have been developed that catalyze …
selectivities. Although artificial metalloenzymes have been developed that catalyze …
Selective CH bond functionalization with engineered heme proteins: new tools to generate complexity
Highlights•Interplay between synthetic chemistry and enzyme discovery/creation.•
Cytochromes P450 for selective hydroxylation of complex molecules.•Engineered heme …
Cytochromes P450 for selective hydroxylation of complex molecules.•Engineered heme …
Genetic optimization of metalloenzymes: enhancing enzymes for non‐natural reactions
Artificial metalloenzymes have received increasing attention over the last decade as a
possible solution to unaddressed challenges in synthetic organic chemistry. Whereas …
possible solution to unaddressed challenges in synthetic organic chemistry. Whereas …
Computation sheds insight into iron porphyrin carbenes' electronic structure, formation, and N–H insertion reactivity
Iron porphyrin carbenes constitute a new frontier of species with considerable synthetic
potential. Exquisitely engineered myoglobin and cytochrome P450 enzymes can generate …
potential. Exquisitely engineered myoglobin and cytochrome P450 enzymes can generate …
Recent developments in the application of P450 based biocatalysts
Highlights•Recent applications of P450 enzymes in natural product biosynthesis.•New
strategies for reconstitution of P450 activity in heterologous hosts.•Creation of new P450 …
strategies for reconstitution of P450 activity in heterologous hosts.•Creation of new P450 …
Unlocked potential of dynamic elements in protein structures: channels and loops
N Kreß, JM Halder, LR Rapp, B Hauer - Current opinion in chemical biology, 2018 - Elsevier
Highlights•Loops and channels are both promising dynamic engineering targets for altered
enzymatic properties.•The relevance of loop dynamics in engineering studies highlights to …
enzymatic properties.•The relevance of loop dynamics in engineering studies highlights to …
Chemogenetic evolution of a peroxidase-like artificial metalloenzyme
Directed evolution has helped enzyme engineering to remarkable successes in the past. A
main challenge in directed evolution is to find the most suitable starting point, that is, an …
main challenge in directed evolution is to find the most suitable starting point, that is, an …