[PDF][PDF] The impact of protein characterization in structural proteomics
A Geerlof, J Brown, B Coutard, MP Egloff… - … Section D: Biological …, 2006 - journals.iucr.org
Protein characterization plays a role in two key aspects of structural proteomics. The first is
the quality assessment of the produced protein preparations. Obtaining well diffracting …
the quality assessment of the produced protein preparations. Obtaining well diffracting …
Ligand-bound structures of the dengue virus protease reveal the active conformation
CG Noble, CC Seh, AT Chao, PY Shi - Journal of virology, 2012 - Am Soc Microbiol
Dengue is a mosquito-borne viral hemorrhagic disease that is a major threat to human
health in tropical and subtropical regions. Here we report crystal structures of a peptide …
health in tropical and subtropical regions. Here we report crystal structures of a peptide …
Lysine methylation as a routine rescue strategy for protein crystallization
TS Walter, C Meier, R Assenberg, KF Au, J Ren… - Structure, 2006 - cell.com
Crystallization remains a critical step in X-ray structure determination. Because it is not
generally possible to rationally predict crystallization conditions, commercial screens have …
generally possible to rationally predict crystallization conditions, commercial screens have …
Space-group and origin ambiguity in macromolecular structures with pseudo-symmetry and its treatment with the program Zanuda
AA Lebedev, MN Isupov - Acta Crystallographica Section D …, 2014 - journals.iucr.org
The presence of pseudo-symmetry in a macromolecular crystal and its interplay with
twinning may lead to an incorrect space-group (SG) assignment. Moreover, if the pseudo …
twinning may lead to an incorrect space-group (SG) assignment. Moreover, if the pseudo …
Higher-throughput approaches to crystallization and crystal structure determination
MJ Fogg, AJ Wilkinson - 2008 - portlandpress.com
In recent times, there has been a large increase in the number of protein structures
deposited in the Protein Data Bank. Structural genomics initiatives have contributed to this …
deposited in the Protein Data Bank. Structural genomics initiatives have contributed to this …
[PDF][PDF] Application of the use of high-throughput technologies to the determination of protein structures of bacterial and viral pathogens
The Structural Proteomics In Europe (SPINE) programme is aimed at the development and
implementation of high-throughput technologies for the efficient structure determination of …
implementation of high-throughput technologies for the efficient structure determination of …
Application of In-Fusion™ Cloning for the Parallel Construction of E. coli Expression Vectors
In-Fusion™ cloning is a flexible DNA ligase-independent cloning technology that has wide-
ranging uses in molecular biology. In this chapter we describe the protocols used in the …
ranging uses in molecular biology. In this chapter we describe the protocols used in the …
A high-throughput cloning system for reverse genetics in Trypanosoma cruzi
Background The three trypanosomatids pathogenic to men, Trypanosoma cruzi,
Trypanosoma brucei and Leishmania major, are etiological agents of Chagas disease …
Trypanosoma brucei and Leishmania major, are etiological agents of Chagas disease …
Semi-automated microseeding of nanolitre crystallization experiments
TS Walter, EJ Mancini, J Kadlec… - … Section F: Structural …, 2008 - journals.iucr.org
A simple semi-automated microseeding procedure for nanolitre crystallization experiments
is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon …
is described. Firstly, a microseed stock solution is made from microcrystals using a Teflon …
Engineering human MEK-1 for structural studies: A case study of combinatorial domain hunting
C Meier, DC Brookings, TA Ceska, C Doyle… - Journal of Structural …, 2012 - Elsevier
Structural biology studies typically require large quantities of pure, soluble protein. Currently
the most widely-used method for obtaining such protein involves the use of bioinformatics …
the most widely-used method for obtaining such protein involves the use of bioinformatics …