Heritable variation in gene expression is common within and between species. This
variation arises from mutations that alter the form or function of molecular gene regulatory …

We are in a phase of unprecedented progress in identifying genetic loci that cause variation
in traits ranging from growth and fitness in simple organisms to disease in humans …

Mutations in non-coding regulatory DNA sequences can alter gene expression, organismal
phenotype and fitness,–. Constructing complete fitness landscapes, in which DNA …

A lack of tools to precisely control gene expression has limited our ability to evaluate
relationships between expression levels and phenotypes. Here, we describe an approach to …

Maximization of growth rate is an important fitness strategy for bacteria. Bacteria can achieve
this by expressing proteins at optimal concentrations, such that resources are not wasted …

Essential genes are the hubs of cellular networks, but lack of high-throughput methods for
titrating gene expression has limited our understanding of the fitness landscapes against …

Data of gene expression levels across individuals, cell types, and disease states is
expanding, yet our understanding of how expression levels impact phenotype is limited …

Antagonistic selection between different fitness components (eg, survival versus fertility) or
different types of individuals in a population (eg, females versus males) can potentially …

The ability to control growth is essential for fundamental studies of bacterial physiology and
biotechnological applications. We have engineered an Escherichia coli strain in which the …

The effects of cell-to-cell variation (noise) in gene expression have proven difficult to quantify
because of the mechanistic coupling of noise to mean expression. To independently …