Applications of next-generation sequencing technologies to diagnostic virology

L Barzon, E Lavezzo, V Militello, S Toppo… - International journal of …, 2011 - mdpi.com
Novel DNA sequencing techniques, referred to as “next-generation” sequencing (NGS),
provide high speed and throughput that can produce an enormous volume of sequences …

Challenges and opportunities in estimating viral genetic diversity from next-generation sequencing data

N Beerenwinkel, HF Günthard, V Roth… - Frontiers in …, 2012 - frontiersin.org
Many viruses, including the clinically relevant RNA viruses HIV (human immunodeficiency
virus) and HCV (hepatitis C virus), exist in large populations and display high genetic …

[HTML][HTML] Next-generation sequencing technology in clinical virology

MR Capobianchi, E Giombini, G Rozera - Clinical Microbiology and …, 2013 - Elsevier
Recent advances in nucleic acid sequencing technologies, referred to as 'next-generation'
sequencing (NGS), have produced a true revolution and opened new perspectives for …

Deep sequencing: becoming a critical tool in clinical virology

ME Quiñones-Mateu, S Avila, G Reyes-Teran… - Journal of clinical …, 2014 - Elsevier
Population (Sanger) sequencing has been the standard method in basic and clinical DNA
sequencing for almost 40 years; however, next-generation (deep) sequencing …

High-throughput sequencing (HTS) for the analysis of viral populations

M Pérez-Losada, M Arenas, JC Galán… - Infection, Genetics and …, 2020 - Elsevier
Abstract The development of High-Throughput Sequencing (HTS) technologies is having a
major impact on the genomic analysis of viral populations. Current HTS platforms can …

Characterization of human immunodeficiency virus type 1 (HIV-1) diversity and tropism in 145 patients with primary HIV-1 infection

P Rieder, B Joos, AU Scherrer, H Kuster… - Clinical infectious …, 2011 - academic.oup.com
Abstract (See the Editorial Commentary by Loes, on pages 1280–2.) Background. In the
context of sexual transmission of human immunodeficiency virus type 1 (HIV-1), current …

Use of four next-generation sequencing platforms to determine HIV-1 coreceptor tropism

J Archer, J Weber, K Henry, D Winner, R Gibson, L Lee… - PloS one, 2012 - journals.plos.org
HIV-1 coreceptor tropism assays are required to rule out the presence of CXCR4-tropic (non-
R5) viruses prior treatment with CCR5 antagonists. Phenotypic (eg, Trofile™, Monogram …

Sensitive deep-sequencing-based HIV-1 genotyping assay to simultaneously determine susceptibility to protease, reverse transcriptase, integrase, and maturation …

RM Gibson, AM Meyer, D Winner, J Archer… - Antimicrobial agents …, 2014 - Am Soc Microbiol
With 29 individual antiretroviral drugs available from six classes that are approved for the
treatment of HIV-1 infection, a combination of different phenotypic and genotypic tests is …

Latent HIV-1 infection occurs in multiple subsets of hematopoietic progenitor cells and is reversed by NF-κB activation

LA McNamara, JA Ganesh, KL Collins - Journal of virology, 2012 - Am Soc Microbiol
The ability of HIV-1 to establish a latent infection presents a barrier to curing HIV. The best-
studied reservoir of latent virus in vivo is resting memory CD4+ T cells, but it has recently …

Evolution of Newcastle disease virus quasispecies diversity and enhanced virulence after passage through chicken air sacs

C Meng, X Qiu, S Yu, C Li, Y Sun, Z Chen, K Liu… - Journal of …, 2016 - Am Soc Microbiol
It has been reported that lentogenic Newcastle disease virus (NDV) isolates have the
potential to become velogenic after their transmission and circulation in chickens, but the …