Structure-based functional mechanisms and biotechnology applications of anti-CRISPR proteins

N Jia, DJ Patel - Nature Reviews Molecular Cell Biology, 2021 - nature.com
CRISPR loci and Cas proteins provide adaptive immunity in prokaryotes against invading
bacteriophages and plasmids. In response, bacteriophages have evolved a broad spectrum …

Anti-CRISPRs: protein inhibitors of CRISPR-Cas systems

AR Davidson, WT Lu, SY Stanley… - Annual review of …, 2020 - annualreviews.org
Clustered regularly interspaced short palindromic repeats (CRISPR) together with their
accompanying cas (CRISPR-associated) genes are found frequently in bacteria and …

Structures and strategies of anti-CRISPR-mediated immune suppression

T Wiegand, S Karambelkar… - Annual review of …, 2020 - annualreviews.org
More than 50 protein families have been identified that inhibit CRISPR (clustered regularly
interspaced short palindromic repeats)-Cas-mediated adaptive immune systems. Here, we …

Diverse mechanisms of CRISPR-Cas9 inhibition by type II anti-CRISPR proteins

S Hwang, KL Maxwell - Journal of Molecular Biology, 2023 - Elsevier
Clustered regularly interspaced short palindromic repeats (CRISPR)-Cas (CRISPR-
associated proteins) systems provide bacteria and archaea with an adaptive immune …

The history of genome editing: advances from the interface of chemistry & biology

D Matsumoto, W Nomura - Chemical Communications, 2023 - pubs.rsc.org
Genome editing had a long history before the appearance of CRISPR. Although a decade
has passed since the initial use of CRISPR with mammalian cells, the first attempts at gene …

Current updates of CRISPR/Cas system and anti-CRISPR proteins: innovative applications to improve the genome editing strategies

KS Allemailem, A Almatroudi, F Alrumaihi… - International Journal …, 2024 - Taylor & Francis
Abstract The Clustered Regularly Interspaced Short Palindromic Repeats
(CRISPR)/CRISPR-associated sequence (CRISPR/Cas) system is a cutting-edge genome …

Optogenetic control of Neisseria meningitidis Cas9 genome editing using an engineered, light-switchable anti-CRISPR protein

MD Hoffmann, J Mathony… - Nucleic acids …, 2021 - academic.oup.com
Optogenetic control of CRISPR–Cas9 systems has significantly improved our ability to
perform genome perturbations in living cells with high precision in time and space. As new …

Anti‐CRISPR proteins targeting the CRISPR‐Cas system enrich the toolkit for genetic engineering

Q Liu, H Zhang, X Huang - The FEBS journal, 2020 - Wiley Online Library
Clustered regularly interspaced short palindromic repeats (CRISPR)‐Cas adaptive immune
defense systems, which are widely distributed in bacteria and Archaea, can provide …

[PDF][PDF] BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II

DH Gu, C Eo, SA Hwangbo, SC Ha… - Journal of …, 2021 - journals.iucr.org
BL-11C, a new protein crystallography beamline, is an in-vacuum undulator-based
microfocus beamline used for macromolecular crystallography at the Pohang Accelerator …

Search for origins of anti-CRISPR proteins by structure comparison

H Sahakyan, KS Makarova, EV Koonin - The CRISPR Journal, 2023 - liebertpub.com
Many bacterial and archaeal viruses encode anti-CRISPR proteins (Acrs) that specifically
inhibit CRISPR-Cas systems via various mechanisms. The majority of the Acrs are small …