Engineered Proteins and Materials Utilizing Residue-Specific Noncanonical Amino Acid Incorporation

T Majekodunmi, D Britton, JK Montclare - Chemical reviews, 2024 - ACS Publications
The incorporation of noncanonical amino acids into proteins and protein-based materials
has significantly expanded the repertoire of available protein structures and chemistries …

[HTML][HTML] Cell-free synthesis of industrial chemicals and biofuels from carbon feedstocks

HJ Lim, DM Kim - Current Opinion in Biotechnology, 2022 - Elsevier
The power of biological systems can be harnessed with higher efficiency when biosynthetic
reactions are decoupled from cellular physiology. This can be achieved by cell-free …

Cell-free protein synthesis systems for vaccine design and production

VT Hu, NP Kamat - Current Opinion in Biotechnology, 2023 - Elsevier
Highlights•CFPS systems allow for highly optimizable and rapid protein production.•CFPS
overcomes limitations in current vaccine production and expands vaccine …

Characterizing and Improving Reaction Times for E. coli-Based Cell-Free Protein Synthesis

LR Burrington, KR Watts, JP Oza - ACS Synthetic Biology, 2021 - ACS Publications
Cell-free protein synthesis (CFPS) is a platform biotechnology that has enabled the on-
demand synthesis of proteins for a variety of applications. Numerous advances have …

Exploring cost reduction strategies for serum free media development

JP Quek, AA Gaffoor, YX Tan, TRM Tan, YF Chua… - npj Science of …, 2024 - nature.com
Cultivated meat production offers solutions in addressing global food security and
sustainability challenges. However, serum-free media (SFM) used in cultivating the cells are …

Vesicle-based cell-free synthesis of short and long unspecific peroxygenases

RM Walter, A Zemella, M Schramm, J Kiebist… - … in bioengineering and …, 2022 - frontiersin.org
Unspecific peroxygenases (UPOs, EC 1.11. 2.1) are fungal enzymes that catalyze the
oxyfunctionalization of non-activated hydrocarbons, making them valuable biocatalysts …

Metabolic Perturbations to an Escherichia coli-based Cell-Free System Reveal a Trade-off between Transcription and Translation

M Kapasiawala, RM Murray - ACS Synthetic Biology, 2024 - ACS Publications
Cell-free transcription–translation (TX–TL) systems have been used for diverse applications,
but their performance and scope are limited by variability and poor predictability. To …

Applications of cell free protein synthesis in protein design

EL Thornton, SM Paterson, MJ Stam, CW Wood… - Protein …, 2024 - Wiley Online Library
In protein design, the ultimate test of success is that the designs function as desired. Here,
we discuss the utility of cell free protein synthesis (CFPS) as a rapid, convenient and …

[HTML][HTML] CT584 Is Not a Protective Vaccine Antigen against Respiratory Chlamydial Challenge in Mice

S Hoang-Phou, S Pal, A Slepenkin… - Vaccines, 2024 - mdpi.com
Background: Chlamydia trachomatis is the most prevalent bacterial sexually transmitted
pathogen in humans worldwide. Since chlamydial infection is largely asymptomatic with the …

Machine learning: an advancement in biochemical engineering

R Saha, A Chauhan, S Rastogi Verma - Biotechnology Letters, 2024 - Springer
One of the most remarkable techniques recently introduced into the field of bioprocess
engineering is machine learning. Bioprocess engineering has drawn much attention due to …