Plasmid copy number and plasmid stability

K Friehs - New trends and developments in biochemical …, 2004 - Springer
Many expression systems in research and industry use plasmids as vectors for the
production of recombinant proteins or non-proteinous recombinant substances. Plasmids …

A brief history of plasmids

DR Helinski - EcoSal Plus, 2022 - Am Soc Microbiol
In the late 1950s, a number of laboratories took up the study of plasmids once the discovery
was made that extrachromosomal antibiotic resistance (R) factors are the responsible …

Type 1 fimbrial expression enhances Escherichia coli virulence for the urinary tract.

I Connell, W Agace, P Klemm… - Proceedings of the …, 1996 - National Acad Sciences
Type 1 fimbriae are adhesion organelles expressed by many Gram-negative bacteria. They
facilitate adherence to mucosal surfaces and inflammatory cells in vitro, but their contribution …

ParE toxin encoded by the broad‐host‐range plasmid RK2 is an inhibitor of Escherichia coli gyrase

Y Jiang, J Pogliano, DR Helinski… - Molecular …, 2002 - Wiley Online Library
Broad‐host‐range plasmid RK2 encodes a post‐segregational killing system, parDE, which
contributes to the stable maintenance of this plasmid in Escherichia coli and many distantly …

Addiction modules and programmed cell death and antideath in bacterial cultures

H Engelberg-Kulka, G Glaser - Annual Reviews in Microbiology, 1999 - annualreviews.org
▪ Abstract In bacteria, programmed cell death is mediated through “addiction modules”
consisting of two genes. The product of the second gene is a stable toxin, whereas the …

[2] Molecular tools for study of biofilm physiology

BB Christensen, C Sternberg, JB Andersen… - Methods in …, 1999 - Elsevier
Publisher Summary This chapter describes methods for the handling and analysis of
microbial behavior of organisms in biofilm communities at both microscopic and …

Prevalence and significance of plasmid maintenance functions in the virulence plasmids of pathogenic bacteria

M Sengupta, S Austin - Infection and immunity, 2011 - Am Soc Microbiol
Virulence functions of pathogenic bacteria are often encoded on large extrachromosomal
plasmids. These plasmids are maintained at low copy number to reduce the metabolic …

Transposons Tn1696 and Tn21and Their Integrons In4 and In2 Have Independent Origins

SR Partridge, HJ Brown, HW Stokes… - Antimicrobial agents and …, 2001 - Am Soc Microbiol
The first 13.6 kb of the mercury and multidrug resistance transposon Tn 1696, which
includes the class 1 integron In4, has been sequenced. In4 is 8.33 kb long and contains the …

Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an …

GY Kholodii, SZ Mindlin, IA Bass… - Molecular …, 1995 - Wiley Online Library
The complete nucleotide sequence of an 8447 bp‐long mercury‐resistance transposon
(Tn5053) has been determined. Tn5053 is composed of two modules:(i) the mercury …

Use of minicircle plasmids for gene therapy

P Mayrhofer, M Schleef, W Jechlinger - Gene Therapy of Cancer: Methods …, 2009 - Springer
A large number of cancer gene therapy clinical trials are currently being performed that are
attempting to evaluate novel approaches to eliminate tumor cells by the introduction of …