Bioinformatics approaches to analyzing CRISPR screen data: from dropout screens to single‐cell CRISPR screens
Y Zhao, M Zhang, D Yang - Quantitative Biology, 2022 - Wiley Online Library
Background Pooled CRISPR screen is a promising tool in drug targets or essential genes
identification with the utilization of three different systems including CRISPR knockout …
identification with the utilization of three different systems including CRISPR knockout …
[HTML][HTML] Single-cell normalization and association testing unifying CRISPR screen and gene co-expression analyses with Normalisr
L Wang - Nature communications, 2021 - nature.com
Single-cell RNA sequencing (scRNA-seq) provides unprecedented technical and statistical
potential to study gene regulation but is subject to technical variations and sparsity …
potential to study gene regulation but is subject to technical variations and sparsity …
The SpliZ generalizes 'percent spliced in'to reveal regulated splicing at single-cell resolution
Detecting single-cell-regulated splicing from droplet-based technologies is challenging.
Here, we introduce the splicing Z score (SpliZ), an annotation-free statistical method to …
Here, we introduce the splicing Z score (SpliZ), an annotation-free statistical method to …
Discovery of target genes and pathways of blood trait loci using pooled CRISPR screens and single cell RNA sequencing
The majority of variants associated with complex traits and common diseases identified by
genome-wide association studies (GWAS) map to noncoding regions of the genome with …
genome-wide association studies (GWAS) map to noncoding regions of the genome with …
[HTML][HTML] Computational identification of clonal cells in single-cell CRISPR screens
Background Single-cell CRISPR screens are powerful tools to understand genome function
by linking genetic perturbations to transcriptome-wide phenotypes. However, since few cells …
by linking genetic perturbations to transcriptome-wide phenotypes. However, since few cells …
CRISPR screens identify gene targets and drug repositioning opportunities at breast cancer risk loci
Genome-wide association studies (GWAS) have identified> 200 loci associated with breast
cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding …
cancer (BC) risk. The majority of candidate causal variants (CCVs) are in non-coding …
Normalisr: normalization and association testing for single-cell CRISPR screen and co-expression
L Wang - bioRxiv, 2021 - biorxiv.org
Single-cell RNA sequencing (scRNA-seq) provides unprecedented technical and statistical
potential to study gene regulation but is subject to technical variations and sparsity. Here we …
potential to study gene regulation but is subject to technical variations and sparsity. Here we …