Escherichia coli is the most researched microbial organism in the world. Its varied impact on
human health, consisting of commensalism, gastrointestinal disease, or extraintestinal …

Enterohemorrhagic Escherichia coli (EHEC) serotype O157: H7 is a food-borne pathogen
that younger children are most prone to this microorganism. Hemolytic Uremic Syndrome …

Conjugate vaccines are among the most effective methods for preventing bacterial
infections. However, existing manufacturing approaches limit access to conjugate vaccines …

Cell-free gene expression (CFE) systems from crude cellular extracts have attracted much
attention for biomanufacturing and synthetic biology. However, activating membrane …

The first, general glycosylation pathway in bacteria, the N-linked glycosylation system of
Campylobacter jejuni, was discovered two decades ago. Since then, many diverse …

Protein glycosylation, the attachment of sugars to amino acid side chains, can endow
proteins with a wide variety of properties of great interest to the engineering biology …

Here we exploit the natural properties of a synthetic nanoparticle (NP) scaffold as a subunit
vaccine against enterohemorrhagic Escherichia coli (EHEC). Two EHEC-specific …

This review is the eighth update of the original article published in 1999 on the application of
Matrix‐assisted laser desorption/ionization mass spectrometry (MALDI) mass spectrometry …

Glycoengineered bacteria have emerged as a cost-effective platform for rapid and
controllable biosynthesis of designer conjugate vaccines. However, little is known about the …

Bacterial glycoconjugate vaccines have a major role in preventing microbial infections.
Immunogenic bacterial glycans, such as O-antigen polysaccharides, can be recombinantly …