Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …

Many biological investigations require 3D imaging of cells or tissues with nanoscale spatial
resolution. We recently discovered that preserved biological specimens can be physically …

Tissue clearing and light-sheet microscopy have a 100-year-plus history, yet these fields
have been combined only recently to facilitate novel experiments and measurements in …

Light-sheet imaging of cleared and expanded samples creates terabyte-sized datasets that
consist of many unaligned three-dimensional image tiles, which must be reconstructed …

High-throughput methods for slide-free three-dimensional (3D) pathological analyses of
whole biopsies and surgical specimens offer the promise of modernizing traditional …

Organisms are inherently three dimensional, thus comprehensive understanding of the
complicated biological system requires analysis of organs or even whole bodies in the …

Light-sheet microscopy is an imaging approach that offers unique advantages for a diverse
range of neuroscience applications. Unlike point-scanning techniques such as confocal and …

We present cleared-tissue axially swept light-sheet microscopy (ctASLM), which enables
isotropic, subcellular resolution imaging with high optical sectioning capability and a large …

Recent advances in optical clearing and light-sheet microscopy have provided
unprecedented access to structural and molecular information from intact tissues. However …

To comprehensively understand tissue and organism physiology and pathophysiology, it is
essential to create complete three-dimensional (3D) cellular maps. These maps require …