Droplet digital PCR of viral‎ DNA/RNA, current progress, challenges, and future perspectives

AA Kojabad, M Farzanehpour… - Journal of Medical …, 2021 - Wiley Online Library
High‐throughput droplet‐based digital PCR (ddPCR) is a refinement of the conventional
polymerase chain reaction (PCR)‎ methods. In ddPCR, DNA/RNA is encapsulated …

Applications of digital PCR for clinical microbiology

J Kuypers, KR Jerome - Journal of clinical microbiology, 2017 - Am Soc Microbiol
Digital PCR (dPCR) is an important new tool for use in the clinical microbiology laboratory.
Its advantages over quantitative PCR (qPCR), including absolute quantification without a …

The challenge of diagnostic metagenomics

AL Greninger - Expert review of molecular diagnostics, 2018 - Taylor & Francis
Introduction: Diagnostic metagenomics and its associated trail of publications are spreading
across the world. Multiple clinical labs in the United States, Europe, and Asia have gone to …

[HTML][HTML] Medical diagnostic value of digital PCR (dPCR): A systematic review

S Nazir - Biomedical Engineering Advances, 2023 - Elsevier
Digital polymerase chain reaction (dPCR) is an emerging technique for the absolute
quantification of target nucleic acids. dPCR got attention as a precise quantification tool in …

Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR

AB Košir, C Divieto, J Pavšič, S Pavarelli… - Analytical and …, 2017 - Springer
Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic
decisions, as overestimation or underestimation can lead to misguided treatment of a …

Quantification of BK virus standards by quantitative real-time PCR and droplet digital PCR is confounded by multiple virus populations in the WHO BKV international …

AC Bateman, AL Greninger, EE Atienza… - Clinical …, 2017 - academic.oup.com
BACKGROUND The WHO recently released a BK virus (BKV) international standard. This
study evaluated the WHO international standard and commercially available BKV standards …

Application of digital PCR in detecting human diseases associated gene mutation

Y Tong, S Shen, H Jiang, Z Chen - Cellular Physiology and …, 2017 - karger.com
Gene mutation has been considered a research hotspot, and the rapid development of
biomedicine has enabled significant advances in the evaluation of gene mutations. The …

A comparison of techniques to evaluate the effectiveness of genome editing

D Germini, T Tsfasman, VV Zakharova, N Sjakste… - Trends in …, 2018 - cell.com
Genome editing using engineered nucleases (meganucleases, zinc finger nucleases,
transcription activator-like effector nucleases) has created many recent breakthroughs …

[HTML][HTML] In vivo disruption of latent HSV by designer endonuclease therapy

M Aubert, EA Madden, M Loprieno, HSDS Feelixge… - JCI insight, 2016 - ncbi.nlm.nih.gov
A large portion of the global population carries latent herpes simplex virus (HSV), which can
periodically reactivate, resulting in asymptomatic shedding or formation of ulcerative lesions …

Characterization of the HIV-1 transcription profile after romidepsin administration in ART-suppressed individuals

S Moron-Lopez, P Kim, OS Søgaard, M Tolstrup… - Aids, 2019 - journals.lww.com
Objectives: Reversing HIV-1 latency has been suggested as a strategy to eradicate HIV-1.
We investigated the effect of romidepsin on the HIV transcription profile in participants from …